{"product_id":"meiob-antibody-meiosis-specific-with-ob-domains-protein-bha17135944","title":"MEIOB Antibody \/ Meiosis-specific with OB domains protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMEIOB Antibody \/ Meiosis-specific with OB domains protein is a anti-MEIOB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MEIOB\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMEIOB antibody detects Meiosis-specific with OB domains protein, a single-stranded DNA-binding protein essential for homologous recombination and meiotic crossover formation. The UniProt recommended name is Meiosis-specific with OB domains protein (MEIOB). This nuclear factor participates in the repair of programmed DNA double-strand breaks (DSBs) during meiosis, ensuring accurate chromosomal segregation and gamete genomic integrity.\u003cbr\u003e\u003cbr\u003eFunctionally, MEIOB antibody identifies a 471-amino-acid protein that localizes to meiotic chromatin in germ cells. MEIOB interacts with single-stranded DNA at resected DSBs and forms a complex with SPATA22, which stabilizes the DNA repair intermediate and promotes strand exchange facilitated by recombinases DMC1 and RAD51. Together, these interactions drive the homologous recombination events that generate genetic diversity during gametogenesis.\u003cbr\u003e\u003cbr\u003eThe MEIOB gene is located on chromosome 16p13.3 and is expressed exclusively in meiotic cells of the testis and ovary. MEIOB contains oligonucleotide\/oligosaccharide-binding (OB) folds characteristic of single-stranded DNA-binding proteins. Its expression begins at the leptotene stage of meiosis and persists through pachytene, where it coordinates crossover formation and synapsis of homologous chromosomes. Loss of MEIOB function results in defective recombination, impaired synaptonemal complex formation, and meiotic arrest.\u003cbr\u003e\u003cbr\u003eIn mammals, MEIOB plays an indispensable role in meiotic DSB repair by stabilizing single-stranded DNA intermediates and promoting strand invasion. It also prevents excessive nuclease activity that could lead to genome instability. In humans, MEIOB mutations have been linked to infertility due to defective gametogenesis, emphasizing its essential contribution to reproductive success. In addition to its germline function, MEIOB expression may serve as a biomarker for spermatogenic activity and ovarian follicle development.\u003cbr\u003e\u003cbr\u003eMEIOB antibody is widely used in reproductive biology, genetics, and DNA repair research. It is suitable for immunohistochemistry, immunoblotting, and chromatin association studies to track MEIOB localization and complex formation. This antibody supports investigations into meiotic recombination, chromosomal synapsis, and genetic stability. It is also used in infertility research to assess meiotic defects and germ cell differentiation.\u003cbr\u003e\u003cbr\u003eStructurally, MEIOB possesses two OB-fold domains for DNA binding and forms a stoichiometric complex with SPATA22 that mimics the RPA-like architecture.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MEIOB - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MEIOB - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MEIOB - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MEIOB - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MEIOB+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310090605,"sku":"FY13042","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f74440be-0e29-438b-8de6-5b56ad6411f7.jpg?v=1782237069","url":"https:\/\/www.ebiohippo.com\/products\/meiob-antibody-meiosis-specific-with-ob-domains-protein-bha17135944","provider":"BioHippo","version":"1.0","type":"link"}