{"product_id":"meltf-antibody-melanotransferrin-mfi2-bha17135846","title":"MELTF Antibody \/ Melanotransferrin \/ MFI2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMELTF Antibody \/ Melanotransferrin \/ MFI2 is a anti-MELTF Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MELTF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMELTF antibody detects Melanotransferrin, a cell-surface glycoprotein related to the transferrin family that participates in iron binding and transport. The UniProt recommended name is Melanotransferrin (MELTF), also known as melanoma-associated antigen p97, melanotransferrin precursor, or MAP97. MELTF was initially identified as a melanoma-associated antigen but is now recognized as a multifunctional iron-binding protein expressed in both normal and malignant cells.\u003cbr\u003e\u003cbr\u003eMELTF antibody targets a glycosylphosphatidylinositol (GPI)-anchored membrane protein of approximately 80 kDa that facilitates cellular iron uptake independent of the classical transferrin receptor pathway. It is expressed in the placenta, intestine, and brain capillaries, where it contributes to iron delivery across epithelial and endothelial barriers. In cancer biology, MELTF is overexpressed in melanomas, glioblastomas, and certain epithelial tumors, where it supports tumor cell proliferation and survival under low-iron conditions. Elevated MELTF expression is associated with increased metastatic potential and angiogenesis through modulation of iron availability.\u003cbr\u003e\u003cbr\u003eThe MELTF antibody is useful in identifying tumor-associated expression and studying iron metabolism mechanisms distinct from transferrin receptor 1 (TFRC) or 2 (TFR2). Structurally, MELTF shares domain homology with transferrins, including two lobes capable of binding ferric iron, but differs by being GPI-anchored rather than secreted. The MELTF gene is located on chromosome 3q13.31 and encodes a 738-amino acid protein that undergoes glycosylation and cleavage to form the mature membrane-associated form. Its regulation is influenced by iron status, hypoxia-inducible factors (HIFs), and oncogenic signaling pathways such as MAPK and PI3K\/AKT.\u003cbr\u003e\u003cbr\u003eRecent studies indicate MELTF may function in neuroprotection by mediating iron uptake in the blood-brain barrier, and mutations or autoantibodies to MELTF have been linked to neurodegenerative disorders. MELTF antibody detection in immunohistochemistry and flow cytometry enables quantification of cell-surface expression, while western blotting reveals glycosylated isoforms. MELTF also plays roles in embryonic development, wound healing, and immune modulation by regulating local iron concentrations.\u003cbr\u003e\u003cbr\u003eAs an iron-binding antigen with diagnostic and therapeutic potential, MELTF antibody supports research in oncology, neurobiology, and iron transport regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MELTF - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MELTF - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MELTF - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MELTF - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MELTF+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306912109,"sku":"FY12944","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1e24c9b5-eca5-4325-a0d7-ac324a91ac4b.jpg?v=1782237068","url":"https:\/\/www.ebiohippo.com\/products\/meltf-antibody-melanotransferrin-mfi2-bha17135846","provider":"BioHippo","version":"1.0","type":"link"}