{"product_id":"membrane-protein-extraction-kit-bht16300166","title":"Membrane Protein Extraction Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eMembrane Protein Extraction\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Membrane Protein Extraction Kit provides 12 extraction solutions for extracting and stabilizing membrane proteins from cell membranes. The solutions are based on different non-denaturing detergents and synthetic lipid analogs: alkyl saccharides (#1, #2), alkylpolyethylenes (#3, #5, #11), acyl-N-methylglucamide (#4), a bile acid salt (#6), an alkylaminoxide (#7), zwitterionic detergents (#8, #9, #12) and a synthetic phosphocholine derivative (#10).\u003c\/p\u003e\n\u003cp\u003eScreening the 12 solutions side by side shows which condition extracts your membrane protein best. The selected solution is also available on its own in a 40 ml size for large-scale extraction (Membrane Protein Extraction Solution).\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eOption\u003c\/th\u003e\n\u003cth\u003eKit\u003c\/th\u003e\n\u003cth\u003eKit Plus\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eMPE01-12S\u003c\/td\u003e\n\u003ctd\u003eMPE01-12P\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSolutions\u003c\/th\u003e\n\u003ctd\u003e12 (#1–#12)\u003c\/td\u003e\n\u003ctd\u003e12 (#1–#12)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eVolume of each solution\u003c\/th\u003e\n\u003ctd\u003e0.5 ml\u003c\/td\u003e\n\u003ctd\u003e5 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUse\u003c\/th\u003e\n\u003ctd\u003eScreening and micro-scale preparative extraction\u003c\/td\u003e\n\u003ctd\u003eScreening and middle-scale preparative extraction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMembrane protein per kit\u003c\/th\u003e\n\u003ctd\u003eUp to 1.5 mg\u003c\/td\u003e\n\u003ctd\u003eUp to 16 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e12 extraction solutions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExtraction agents\u003c\/th\u003e\n\u003ctd\u003eNon-denaturing detergents and synthetic lipid analogs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eMPE01-12S, MPE01-12P\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003ePrepare cell membranes (for example by high-speed centrifugation of the cell lysate), and place membranes containing about 0.2–0.3 mg of membrane protein in 12 tubes.\u003c\/li\u003e\n\u003cli\u003ePellet the membranes at 100,000 x g for 45 min and remove the supernatant.\u003c\/li\u003e\n\u003cli\u003eAdd 100 µl of each extraction solution (with protease inhibitors) to a different tube, suspend the membranes well and incubate.\u003c\/li\u003e\n\u003cli\u003eSeparate the extracted proteins from the insoluble material and compare the extraction efficiency of the 12 solutions.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe ProFoldin protocol “Membrane Protein Extraction” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eCatalog #s MPE01-12S (Kit) and MPE01-12P (Kit Plus) contain all 12 solutions for screening. Catalog #s MPE01 to MPE12 are single 40 ml bottles of one solution (Membrane Protein Extraction Solution).\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Kit (12 solutions × 0.5 ml)","offer_id":67828319519085,"sku":"MPE01-12S","price":216.0,"currency_code":"USD","in_stock":true},{"title":"Kit Plus (12 solutions × 5 ml)","offer_id":67828321714541,"sku":"MPE01-12P","price":471.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/MPE01-12S_1.jpg?v=1793129658","url":"https:\/\/www.ebiohippo.com\/products\/membrane-protein-extraction-kit-bht16300166","provider":"BioHippo","version":"1.0","type":"link"}