{"product_id":"mepce-antibody-7sk-snrna-methylphosphate-capping-enzyme-bha17136056","title":"MEPCE Antibody \/ 7SK snRNA methylphosphate capping enzyme","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMEPCE Antibody \/ 7SK snRNA methylphosphate capping enzyme is a anti-MEPCE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MEPCE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMEPCE antibody detects 7SK snRNA methylphosphate capping enzyme, a nuclear RNA methyltransferase that stabilizes 7SK small nuclear RNA and regulates transcription elongation. The UniProt recommended name is 7SK snRNA methylphosphate capping enzyme (MEPCE). This enzyme catalyzes the methylation of the 5? gamma-phosphate of 7SK snRNA, forming a protective cap structure critical for RNA stability.\u003cbr\u003e\u003cbr\u003eFunctionally, MEPCE antibody identifies a 689-amino-acid enzyme that binds to 7SK snRNA within the 7SK ribonucleoprotein complex. MEPCE cooperates with LARP7 and HEXIM1 to maintain repression of positive transcription elongation factor b (P-TEFb), thereby controlling RNA polymerase II elongation and global transcriptional output. It serves as a key regulator of gene expression homeostasis.\u003cbr\u003e\u003cbr\u003eThe MEPCE gene is located on chromosome 7q22.3 and is ubiquitously expressed, with enrichment in transcriptionally active tissues such as brain, liver, and gonads. MEPCE function is essential for normal development and maintenance of chromatin transcription dynamics.\u003cbr\u003e\u003cbr\u003ePathologically, mutations or reduced expression of MEPCE cause neurodevelopmental disorders characterized by intellectual disability and seizures. Disruption of 7SK complex stability leads to uncontrolled transcription elongation and altered gene expression. Research using MEPCE antibody supports studies in RNA biology, transcription regulation, and neurological disease mechanisms.\u003cbr\u003e\u003cbr\u003eMEPCE antibody is validated for western blotting, immunoprecipitation, and immunofluorescence to detect RNA methyltransferases and transcriptional regulators. \u003cbr\u003e\u003cbr\u003eStructurally, 7SK snRNA methylphosphate capping enzyme contains a conserved methyltransferase domain that binds S-adenosylmethionine and the 7SK RNA substrate. This antibody facilitates investigation of MEPCE's enzymatic role in transcriptional repression and RNA stability.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MEPCE - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MEPCE - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MEPCE - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MEPCE - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MEPCE+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313891693,"sku":"FY13154","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f8e796dc-47ae-4e5b-aad7-6f4cc7990c4a.jpg?v=1782237099","url":"https:\/\/www.ebiohippo.com\/products\/mepce-antibody-7sk-snrna-methylphosphate-capping-enzyme-bha17136056","provider":"BioHippo","version":"1.0","type":"link"}