{"product_id":"mest-antibody-mesoderm-specific-transcript-protein-bha17135849","title":"MEST Antibody \/ Mesoderm-specific transcript protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMEST Antibody \/ Mesoderm-specific transcript protein is a anti-MEST Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MEST\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMEST antibody detects Mesoderm-specific transcript protein, an imprinted gene product associated with growth, differentiation, and placental development. The UniProt recommended name is Mesoderm-specific transcript protein (MEST), with alternate names including paternally expressed gene 1 (PEG1) and alpha\/beta hydrolase domain-containing protein 14C. MEST belongs to the alpha\/beta hydrolase superfamily and is expressed predominantly from the paternal allele, exhibiting tissue-specific imprinting patterns.\u003cbr\u003e\u003cbr\u003eFunctionally, MEST antibody identifies a 335-amino-acid protein that contains a predicted hydrolase fold and is implicated in adipocyte differentiation, embryonic growth, and metabolic regulation. Although its enzymatic substrate remains uncertain, structural homology suggests esterase or lipase-like activity. MEST is expressed during mesoderm formation and plays a key role in development of the placenta, skeletal muscle, and adipose tissue. Elevated MEST expression correlates with obesity, insulin resistance, and altered lipid storage, suggesting a regulatory role in adipogenesis and energy metabolism.\u003cbr\u003e\u003cbr\u003eThe MEST antibody is used to study genomic imprinting, epigenetic regulation, and developmental gene expression. MEST is a key marker for parent-of-origin gene expression studies, given that it is expressed exclusively from the paternal allele in most tissues. The MEST gene is located on chromosome 7q32.2 and lies within an imprinted domain regulated by differential DNA methylation. Aberrant methylation or loss of imprinting at this locus has been associated with developmental disorders such as Silver-Russell syndrome and growth abnormalities.\u003cbr\u003e\u003cbr\u003eIn adult tissues, MEST expression is detected in adipocytes, brain, and pancreas, where it influences energy balance and metabolic plasticity. Research suggests that MEST interacts with Wnt signaling pathways to regulate cell fate determination and tissue remodeling. In cancer, MEST dysregulation has been linked to metastasis and epithelial-to-mesenchymal transition (EMT). Overexpression of MEST promotes cell migration and invasion, while silencing reduces tumor aggressiveness.\u003cbr\u003e\u003cbr\u003eMEST antibody applications include western blotting, immunofluorescence, and RT-qPCR normalization in imprinting and developmental biology research. Because of its epigenetic regulation, MEST serves as a valuable biomarker for studying parental imprinting effects and obesity-related gene expression.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MEST - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MEST - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MEST - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MEST - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MEST+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306813805,"sku":"FY12947","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0a44759d-d2cd-4f10-9be9-7ca2e625cb84.jpg?v=1782237064","url":"https:\/\/www.ebiohippo.com\/products\/mest-antibody-mesoderm-specific-transcript-protein-bha17135849","provider":"BioHippo","version":"1.0","type":"link"}