{"product_id":"mfsd2a-antibody-major-facilitator-superfamily-domain-containing-protein-2a-bha17135466","title":"MFSD2A Antibody \/ Major facilitator superfamily domain-containing protein 2A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMFSD2A Antibody \/ Major facilitator superfamily domain-containing protein 2A is a anti-MFSD2A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Punctate cytoplasmic, ER, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MFSD2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMFSD2A antibody detects Major facilitator superfamily domain-containing protein 2A, a sodium-dependent lysophosphatidylcholine symporter that mediates the uptake of docosahexaenoic acid (DHA) into the brain and retina. MFSD2A plays a critical role in maintaining the blood-brain barrier (BBB) and enabling lipid transport across endothelial cells. The MFSD2A antibody is widely used in neuroscience, vascular biology, and metabolic research to investigate BBB integrity, lipid trafficking, and neurodevelopmental regulation.\u003cbr\u003e\u003cbr\u003eMFSD2A is encoded by the MFSD2A gene located on human chromosome 1p34.2. The protein belongs to the major facilitator superfamily of solute carriers and is approximately 530 amino acids in length, with 12 predicted transmembrane helices. MFSD2A localizes to the plasma membrane of endothelial cells lining brain capillaries, where it functions as a transporter for lysophosphatidylcholine-bound polyunsaturated fatty acids. Unlike most nutrient transporters at the BBB, MFSD2A is not energy-dependent but couples lipid import to sodium gradients.\u003cbr\u003e\u003cbr\u003eThe MFSD2A antibody detects a 62 kilodalton band in western blot and demonstrates strong vascular endothelial staining in brain tissue and retina. In mouse models, deletion of MFSD2A results in severe microcephaly, neuronal loss, and impaired synaptic development due to reduced DHA delivery to the brain. In adults, MFSD2A maintains BBB tightness by suppressing vesicular transcytosis, ensuring selective permeability and protecting the central nervous system from toxins and immune cell infiltration.\u003cbr\u003e\u003cbr\u003eMFSD2A expression is regulated by pericytes and Wnt\/beta-catenin signaling pathways, linking vascular maturation to lipid metabolism. Its dysfunction has been associated with neurodevelopmental disorders, cognitive impairment, and visual defects. In cancer research, MFSD2A acts as a metastasis suppressor by inhibiting vascular leakage and tumor cell extravasation, demonstrating a dual role in nutrient transport and vascular homeostasis.\u003cbr\u003e\u003cbr\u003eBeyond the nervous system, MFSD2A participates in lipid absorption in the placenta and peripheral vasculature. Because of its essential function in brain DHA uptake, MFSD2A is a promising target for therapeutic strategies aimed at enhancing neuroprotection and treating BBB disorders.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MFSD2A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MFSD2A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MFSD2A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MFSD2A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MFSD2A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293641069,"sku":"FY12563","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3f72a1c3-83fa-49bf-a1b6-77c68e3ca4b9.jpg?v=1782237031","url":"https:\/\/www.ebiohippo.com\/products\/mfsd2a-antibody-major-facilitator-superfamily-domain-containing-protein-2a-bha17135466","provider":"BioHippo","version":"1.0","type":"link"}