{"product_id":"mib2-antibody-mindbomb-2-bha17136298","title":"MIB2 Antibody \/ Mindbomb 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMIB2 Antibody \/ Mindbomb 2 is a anti-MIB2 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MIB2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMIB2 (Mindbomb 2) is a RING-type E3 ubiquitin ligase that regulates membrane protein turnover and signal transduction, most notably within the Notch pathway. By catalyzing ubiquitination of Delta-like ligands, MIB2 promotes their endocytosis and activation competence, ensuring proper receptor engagement and downstream transcriptional responses. Through these activities, MIB2 helps coordinate cell–cell communication during development and tissue homeostasis.\u003cp\u003eBeyond canonical Notch signaling, MIB2 has been implicated in cytoskeletal organization and broader ubiquitin-mediated quality control. Altered MIB2 expression or activity can influence cell fate decisions, neural and immune system function, and tumor biology, where disrupted ligand trafficking and signaling balance contribute to disease phenotypes. Its functional overlap and divergence relative to the paralog MIB1 highlight MIB2 as a distinct regulator with tissue- and context-specific roles.\u003c\/p\u003e\n\u003cp\u003eThe MIB2 antibody enables specific detection of endogenous MIB2 in cells and tissues for applications including western blot, immunofluorescence, and immunohistochemistry. Researchers use the MIB2 antibody to quantify expression, assess subcellular localization at membranes and endosomes, and monitor pathway modulation under genetic or pharmacologic perturbations. With high specificity and consistent performance, the MIB2 antibody supports studies of ubiquitin signaling, Notch pathway regulation, and disease mechanisms.\u003c\/p\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MIB2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MIB2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MIB2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MIB2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MIB2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047325426029,"sku":"RQ8879","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_81fecc82-e9b8-42e1-9059-9e28e3fddc49.jpg?v=1772019439","url":"https:\/\/www.ebiohippo.com\/products\/mib2-antibody-mindbomb-2-bha17136298","provider":"BioHippo","version":"1.0","type":"link"}