{"product_id":"mical3-antibody-molecule-interacting-with-casl-protein-3-bha17135689","title":"MICAL3 Antibody \/ Molecule interacting with CasL protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMICAL3 Antibody \/ Molecule interacting with CasL protein 3 is a anti-MICAL3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MICAL3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMICAL3 antibody detects Microtubule-associated monooxygenase, calponin, and LIM domain-containing protein 3, an actin-modifying enzyme that regulates cytoskeletal organization and vesicle trafficking. Encoded by the MICAL3 gene on chromosome 22q11.21, this multidomain oxidoreductase functions downstream of Rab GTPases to control exocytosis and vesicle docking at the plasma membrane. MICAL3 is part of the MICAL protein family, characterized by an N-terminal flavoprotein monooxygenase domain, calponin homology domain, LIM domain, and coiled-coil regions that mediate interactions with cytoskeletal and vesicular components.\u003cbr\u003e\u003cbr\u003eMICAL3 catalyzes F-actin oxidation, leading to filament disassembly and cytoskeletal remodeling required for vesicle fusion. It interacts with Rab8A and Rab13, forming complexes that regulate polarized exocytosis and membrane trafficking in epithelial cells. MICAL3 is localized to vesicular membranes and actin-rich regions of the cytoplasm, where it coordinates actin depolymerization and vesicle release. Its enzymatic activity is dependent on flavin adenine dinucleotide (FAD) and modulates actin dynamics through redox reactions on specific methionine residues.\u003cbr\u003e\u003cbr\u003eThe MICAL3 antibody is used in cell biology, neurobiology, and membrane trafficking research to study cytoskeletal regulation and exocytosis. Western blot analysis identifies a 230 kilodalton band corresponding to MICAL3, while immunofluorescence reveals punctate cytoplasmic staining in vesicle-associated regions. This antibody enables investigation of actin redox control, vesicle tethering, and neuronal growth cone dynamics.\u003cbr\u003e\u003cbr\u003eMICAL3 has been implicated in axonal transport, endocytosis, and cancer cell migration. Its deregulation may contribute to disorders involving vesicle trafficking or cytoskeletal disorganization. The MICAL3 antibody provides a validated tool for studying redox-regulated actin remodeling and membrane dynamics.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MICAL3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MICAL3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MICAL3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MICAL3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MICAL3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299735917,"sku":"FY12787","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a924d9c9-7624-4861-8e62-79f8c6613887.jpg?v=1782237037","url":"https:\/\/www.ebiohippo.com\/products\/mical3-antibody-molecule-interacting-with-casl-protein-3-bha17135689","provider":"BioHippo","version":"1.0","type":"link"}