{"product_id":"micro-phosphate-removal-column-set-bhu16300006","title":"Micro Phosphate Removal Column Set","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eSpin Columns\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Micro Phosphate Removal Column Set removes inorganic phosphate from buffer solutions. Phosphate binds to the column resin and stays on the column, while regular buffer salts and non-charged molecules such as sugars and glycerol do not bind and stay in the sample.\u003c\/p\u003e\n\u003cp\u003eSome organic phosphates such as ATP also bind to the resin, but less strongly than inorganic phosphate. Metal chelators such as EDTA interfere with phosphate binding to the resin. Removing phosphate is useful, for example, before phosphatase activity assays.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e20 columns – MPR020\u003c\/th\u003e\n\u003ctd\u003e20 pre-packed spin columns.\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003ePre-packed spin columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003ctd\u003e20 columns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Volume\u003c\/th\u003e\n\u003ctd\u003e200 µl per column\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eResin Binding Capacity\u003c\/th\u003e\n\u003ctd\u003e200 µmol phosphate per ml of bed volume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRemoval per Column (0.2 ml sample)\u003c\/th\u003e\n\u003ctd\u003e500 mM to 3 mM; 350 mM to 0.5 mM; 175 mM to 0.2 mM; 10 mM to 0.0003 mM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEquipment Needed\u003c\/th\u003e\n\u003ctd\u003eBench-top microcentrifuge and 1.5 ml microcentrifuge tubes as receiver tubes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eMPR020\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped cold (not frozen).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eSpin each column briefly to settle the resin, remove the bottom tip and cap, and spin twice at 13,000 rpm for 1 min so the resin is almost dry.\u003c\/li\u003e\n\u003cli\u003eMove the column to a clean 1.5 ml tube, load 200 µl of sample, spin at 1,000 rpm for 1 min and then at 13,000 rpm for 1 min, and keep the eluate.\u003c\/li\u003e\n\u003cli\u003eSpin the eluate at 13,000 rpm for 1 min, or filter it through a 0.22 µm filter, to remove any insoluble material.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe full ProFoldin protocol “Micro Phosphate Removal Column Set” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eAvoid EDTA and other metal chelators in the sample; they interfere with phosphate binding to the resin.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"20 columns","offer_id":67815923384685,"sku":"MPR020","price":310.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/MPR020_copy.jpg?v=1793049625","url":"https:\/\/www.ebiohippo.com\/products\/micro-phosphate-removal-column-set-bhu16300006","provider":"BioHippo","version":"1.0","type":"link"}