{"product_id":"micromolar-phosphate-assay-reagent-bht16300173","title":"MicroMolar Phosphate Assay Reagent","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eAssay Reagents\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe MicroMolar Phosphate Assay Reagent measures 0.2–20 µM inorganic phosphate. Phosphate reacts with molybdate and forms a blue complex with malachite green dye in acidic solution, and the amount of phosphate is measured by light absorbance at 650 nm.\u003c\/p\u003e\n\u003cp\u003eEach pack contains 30 ml of reagent, enough for 200 assays in 96-well plates or more than 600 assays in 384-well plates. The reagent can also be used in cuvettes with a spectrophotometer.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e200 assays (30 ml) – MPA200\u003c\/th\u003e\n\u003ctd\u003e30 ml of assay reagent for 200 assays in 96-well plates or more than 600 assays in 384-well plates\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection range\u003c\/th\u003e\n\u003ctd\u003e0.2–20 µM phosphate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection\u003c\/th\u003e\n\u003ctd\u003eAbsorbance at 650 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eVolume\u003c\/th\u003e\n\u003ctd\u003e30 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssays\u003c\/th\u003e\n\u003ctd\u003e200 (96-well) or more than 600 (384-well)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eMPA200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped at room temperature.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003ePrepare phosphate standards from 0.2 µM to 20 µM.\u003c\/li\u003e\n\u003cli\u003eIn a 96-well plate, mix 100 µl of standard or sample with 150 µl of the reagent and incubate for 5 min.\u003c\/li\u003e\n\u003cli\u003eRead absorbance at 650 nm, plot the standard curve and calculate the phosphate concentration of the samples.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe ProFoldin protocol “Phosphate Assay” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"200 assays (30 ml)","offer_id":67828368277869,"sku":"MPA200","price":157.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/micromolar-phosphate-assay-reagent-bht16300173","provider":"BioHippo","version":"1.0","type":"link"}