{"product_id":"micromolar-universal-kinase-assay-kit-bht16300113","title":"MicroMolar Universal Kinase Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eEnzyme Activity Assay Kit\u003c\/span\u003e\u003cp\u003eKinases transfer a phosphate from ATP to a substrate, giving a phosphorylated substrate and ADP. Measuring the ADP formed is a general way to follow the activity of purified kinases regardless of the substrate.\u003c\/p\u003e\n\u003ch2\u003ePack Sizes\u003c\/h2\u003e\n\u003cp\u003eBoth sizes contain the same reagents. The only difference is the number of assays: MUK100K is for 100 assays and MUK1000K for 1000 assays (384-well format); see the Kit Components table for the volumes of each size.\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003e100 assays (MUK100K)\u003c\/th\u003e\n\u003ctd\u003e100 assays; enzyme not included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003e1000 assays (MUK1000K)\u003c\/th\u003e\n\u003ctd\u003e1000 assays; enzyme not included\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e100 assays (MUK100K)\u003c\/th\u003e\n\u003cth\u003e1000 assays (MUK1000K)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Assay buffer\u003c\/td\u003e\n\u003ctd\u003e600 µl\u003c\/td\u003e\n\u003ctd\u003e4000 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x ATP\u003c\/td\u003e\n\u003ctd\u003e35 µl\u003c\/td\u003e\n\u003ctd\u003e350 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x MUK Reagent A\u003c\/td\u003e\n\u003ctd\u003e35 µl\u003c\/td\u003e\n\u003ctd\u003e350 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x MUK Reagent B\u003c\/td\u003e\n\u003ctd\u003e35 µl\u003c\/td\u003e\n\u003ctd\u003e350 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1 mM ADP\u003c\/td\u003e\n\u003ctd\u003e30 µl\u003c\/td\u003e\n\u003ctd\u003e200 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Fluorescence dye\u003c\/td\u003e\n\u003ctd\u003e350 µl\u003c\/td\u003e\n\u003ctd\u003e3500 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eThe 1 x MUK Buffer is 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 3 mM MgCl2, 0.5 mM DTT, 0.2 mM EDTA, 0.003% Brij-35. Kinase and kinase substrate are supplied by the user.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThe kit measures the ADP produced by the kinase reaction. MUK Reagents A and B are added after a 2 min pre-incubation and are present during the 60 min reaction; a fluorescence dye is then added to read the ADP signal at 535 nm (excitation 485 nm). Low ATP (20 µM recommended) keeps the ADP background low and lets ATP-competitive inhibitors be detected.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eKinase activity (ADP formation)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Type\u003c\/th\u003e\n\u003ctd\u003eKinase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eEnzyme Activity Assay; Inhibitor Screening Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Wavelength\u003c\/th\u003e\n\u003ctd\u003eEx 485 nm \/ Em 535 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Format\u003c\/th\u003e\n\u003ctd\u003e384\/96-well plate or micro-cuvette\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eInstrument Required\u003c\/th\u003e\n\u003ctd\u003ePlate reader or fluorometer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Time\u003c\/th\u003e\n\u003ctd\u003eAbout 62 min (2 min pre-incubation + 60 min reaction)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMeasuring Range\u003c\/th\u003e\n\u003ctd\u003e0.1 µM to 10 µM ADP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecommended ATP\u003c\/th\u003e\n\u003ctd\u003e20 µM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e100 assays, 1000 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eThe manufacturer's protocol describes the procedure and reaction volumes.\u003c\/p\u003e\n\u003cul\u003e\u003cli\u003eUniversal Kinase Assay (PDF, see Documents): Covers catalog # MUK100K, MUK1000K.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The MicroMolar Universal Kinase Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"100 assays","offer_id":67794880168301,"sku":"MUK100K","price":513.0,"currency_code":"USD","in_stock":true},{"title":"1000 assays","offer_id":67794884493677,"sku":"MUK1000K","price":2884.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/MUK_copy.jpg?v=1792791969","url":"https:\/\/www.ebiohippo.com\/products\/micromolar-universal-kinase-assay-kit-bht16300113","provider":"BioHippo","version":"1.0","type":"link"}