{"product_id":"mid2-antibody-midline-2-bha17135631","title":"MID2 Antibody \/ Midline 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMID2 Antibody \/ Midline 2 is a anti-MID2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MID2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMID2 antibody detects E3 ubiquitin-protein ligase MID2 (also known as Midline-2 or FXY2), a microtubule-associated E3 ligase involved in cytoskeletal organization, cell polarity, and developmental morphogenesis. Encoded by the MID2 gene on chromosome Xq22.3, this protein belongs to the tripartite motif (TRIM) family of ubiquitin ligases, featuring a RING finger domain, B-box zinc-binding motifs, a coiled-coil region, and a C-terminal COS domain that binds microtubules. MID2 functions in ubiquitination of cytoskeletal and signaling proteins, coordinating cell structure and trafficking processes crucial for morphogenesis and neurodevelopment.\u003cbr\u003e\u003cbr\u003eIn human cells, MID2 localizes along microtubules, at the centrosome, and in perinuclear regions. It associates with its close paralog MID1, with which it shares structural and functional similarities. Together, MID1 and MID2 regulate the stability of PP2A catalytic subunits and participate in tubulin network maintenance. MID2 expression is particularly high in brain, testis, and embryonic tissues, reflecting roles in neuronal migration and organ development. Mutations in MID2 have been associated with X-linked intellectual disability and developmental disorders affecting craniofacial and skeletal structures.\u003cbr\u003e\u003cbr\u003eThe MID2 antibody is widely used in cell biology and developmental research to study microtubule organization, ubiquitination, and cell morphology. Western blot analysis detects a ~78 kilodalton band corresponding to full-length MID2, while immunofluorescence reveals filamentous staining patterns overlapping with microtubules. MID2 ubiquitinates several substrates involved in cytoskeletal remodeling and signaling, including MID1-associated proteins and small GTPase regulators. Through these activities, it influences cell polarity, division, and migration. In neurons, MID2 contributes to axon guidance and growth cone dynamics.\u003cbr\u003e\u003cbr\u003eDysregulation of MID2 expression or mutation of its functional domains can lead to developmental abnormalities and neurological defects. Overexpression has been observed in certain cancers, where it may enhance cell motility and invasiveness through altered cytoskeletal signaling. The MID2 antibody provides a robust tool for investigating these pathways and identifying mechanisms of microtubule regulation in both normal and diseased states.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MID2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MID2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MID2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MID2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MID2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298457965,"sku":"FY12728","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fc87aea7-64a4-4dce-bfc5-0b32b89ec19f.jpg?v=1782237043","url":"https:\/\/www.ebiohippo.com\/products\/mid2-antibody-midline-2-bha17135631","provider":"BioHippo","version":"1.0","type":"link"}