{"product_id":"mir-29a-3p-gfp-puro-bhv19400176","title":"miR-29a-3p-GFP-Puro","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003emiR-29a-3p is the mature microRNA derived from the MIR29A stem-loop and is conserved between human and mouse. It is produced through the canonical microRNA biogenesis pathway, in which a primary transcript is processed by Drosha\/DGCR8 and Dicer and the mature strand is loaded into the RNA-induced silencing complex to repress target mRNAs. The miR-29 family is well known for targeting extracellular matrix genes, including multiple collagens, as well as DNA methyltransferases, linking it to tissue fibrosis and epigenetic regulation. Through this target network, miR-29a-3p has been studied in fibrotic disease, cell differentiation, and as a frequently downregulated, tumor-suppressive microRNA in cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis miRNA lentivirus delivers the complete human miR-29a stem-loop, which is processed in transduced cells to yield the mature miR-29a-3p sequence common to human and mouse. The stem-loop is driven by a U6 promoter, while a PGK promoter drives a GFP reporter and a puromycin selection marker separated by a self-cleaving peptide, so transduced cells can be identified by fluorescence and enriched by antibiotic selection or FACS.\u003c\/p\u003e\n\u003cp\u003eThe particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation, supporting in vitro and in vivo use and transduction of difficult-to-transfect cells such as primary and thawed cultures. Polyclonal stable cell lines can be established within about 10 days for long-term studies of miR-29a function, target repression, and microRNA biology while preserving parental cell properties.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis lentivirus expresses the full pre-miRNA stem-loop sequence of \u003cstrong\u003eMIR29A\u003c\/strong\u003e under a Pol II promoter (CMV or EF1α), enabling endogenous Drosha\/DGCR8 and Dicer processing to produce authentic mature 3P (and 5p) strands loaded into RISC. This produces physiologically relevant miRNA activity that faithfully replicates the endogenous biogenesis pathway, unlike synthetic miRNA mimics that bypass nuclear processing and can exhibit RISC overloading artifacts.\u003c\/p\u003e\n\u003cp\u003eCo-expression of a fluorescent reporter (GFP) and selection marker (Puromycin) allows stable transductant identification by fluorescence and antibiotic enrichment. Stable miRNA overexpression enables long-term target repression studies, in vivo tumor models, and exosomal miRNA loading experiments that are not achievable with transient miRNA mimics.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 1x10^6","offer_id":53251620700525,"sku":"LMV-0003-1S","price":595.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/mir-29a-3p-gfp-puro-bhv19400176","provider":"BioHippo","version":"1.0","type":"link"}