{"product_id":"mir-375-3p-gfp-puro-bhv19400177","title":"miR-375-3p-GFP-Puro","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003emiR-375 is a conserved microRNA that post-transcriptionally regulates gene expression by guiding the RNA-induced silencing complex to complementary sequences in target mRNAs, leading to translational repression or transcript destabilization. miR-375 is highly enriched in pancreatic islets, where it influences insulin secretion and beta-cell function, and it is also expressed in epithelial and neuroendocrine tissues. It frequently behaves as a tumor-suppressive microRNA, with altered expression reported across several carcinomas. Through these activities, miR-375 contributes to endocrine regulation and cancer biology and is widely studied as a functional and biomarker microRNA.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe miR-375-3p-GFP-Puro Lentivirus delivers stable overexpression of human and mouse miR-375. The vector carries the entire miRNA stem-loop, allowing endogenous Drosha and Dicer processing to generate the authentic mature miR-375-3p strand for loading into the RNA-induced silencing complex. The stem-loop is driven by a U6 promoter, while a PGK promoter drives a GFP fluorescent reporter and a puromycin selection marker separated by a self-cleaving peptide. Transduced cells are readily identified and enriched by GFP fluorescence or puromycin selection, with polyclonal stable lines established within about ten days while preserving parental cell properties. It is used for long-term target-repression studies, tumor models, and other functional microRNA experiments. Supplied as ultra-purified, high-titer lentiviral particles suitable for in vitro and in vivo use and for primary or thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis lentivirus expresses the full pre-miRNA stem-loop sequence of \u003cstrong\u003eMIR375\u003c\/strong\u003e under a Pol II promoter (CMV or EF1α), enabling endogenous Drosha\/DGCR8 and Dicer processing to produce authentic mature 3P (and 5p) strands loaded into RISC. This produces physiologically relevant miRNA activity that faithfully replicates the endogenous biogenesis pathway, unlike synthetic miRNA mimics that bypass nuclear processing and can exhibit RISC overloading artifacts.\u003c\/p\u003e\n\u003cp\u003eCo-expression of a fluorescent reporter (GFP) and selection marker (Puromycin) allows stable transductant identification by fluorescence and antibiotic enrichment. Stable miRNA overexpression enables long-term target repression studies, in vivo tumor models, and exosomal miRNA loading experiments that are not achievable with transient miRNA mimics.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 1x10^6","offer_id":53251620274541,"sku":"LMV-0005-1S","price":595.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/mir-375-3p-gfp-puro-bhv19400177","provider":"BioHippo","version":"1.0","type":"link"}