{"product_id":"mirna-purification-kit-bht15200024","title":"miRNA Purification Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe miRNA extraction kit is specially used to separate and purify miRNA from various animal tissues, plant tissues, cells, serum, plasma and other samples. It can also extract siRNA, snRNA and other small molecular RNAs less than 200 nt as well as total RNA. This product combines phenol\/guanidine lysis technology and silica matrix membrane purification technology. Its unique lysis solution can effectively inhibit RNases while removing most of the DNA and protein in cells or tissue samples by organic extraction. This product can be directly used for Northern Blot analysis, Real-Time PCR, Microarray Analysis, etc.\u003c\/p\u003e\n\u003cp\u003eTwo protocols are supplied, one for miRNA enrichment and one for total RNA extraction. The general workflow lyses tissue or cells with TRIzon Reagent, extracts with chloroform, precipitates with ethanol, binds and washes on the supplied spin columns and elutes in 30–50 µL of RNase-Free Water. All centrifugation is carried out at 12,000 rpm at room temperature, and a drying step precedes elution to remove residual ethanol that would otherwise affect enzymatic reactions.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin columns RM and RS with TRIzon Reagent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eChemistry\u003c\/th\u003e\n\u003ctd\u003ePhenol\/guanidine lysis combined with silica matrix membrane purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003eAnimal tissues, plant tissues, cells, serum and plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNucleic acids recovered\u003c\/th\u003e\n\u003ctd\u003emiRNA, siRNA, snRNA and other small RNAs less than 200 nt; total RNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtocols supplied\u003c\/th\u003e\n\u003ctd\u003emiRNA enrichment and total RNA extraction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e30–50 µL RNase-Free Water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTRIzon Reagent\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RWT (concentrate)\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW2 (concentrate)\u003c\/td\u003e\n\u003ctd\u003e11 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free Water\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpin Columns RM with Collection Tubes\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpin Columns RS with Collection Tubes\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free Centrifuge Tubes (1.5 mL)\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003emiRNA separation and purification from tissues, cells, serum and plasma\u003c\/li\u003e\n\u003cli\u003eExtraction of siRNA, snRNA and other small RNAs less than 200 nt\u003c\/li\u003e\n\u003cli\u003eTotal RNA extraction\u003c\/li\u003e\n\u003cli\u003eNorthern Blot analysis and Real-Time PCR\u003c\/li\u003e\n\u003cli\u003eMicroarray analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eTRIzon Reagent is stored at 2–8°C in the dark; the other components are stored at room temperature (15–30°C). The shelf life is 1 year at room temperature.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUse RNase-free plastics, autoclaved glassware and RNase-free water to prevent RNase contamination.\u003c\/li\u003e\n\u003cli\u003eWear a mask and gloves during the procedure.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze-thaw cycles.\u003c\/li\u003e\n\u003cli\u003eAdd ethanol to the concentrated buffers before first use.\u003c\/li\u003e\n\u003cli\u003eDo not omit the drying step, as residual ethanol affects enzymatic reactions.\u003c\/li\u003e\n\u003cli\u003eChloroform and ethanol are required but are not supplied.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP028\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200024\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965512507757,"sku":"EP028-50T","price":390.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965517521261,"sku":"EP028-50TX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP028-a-1.jpg?v=1789419121","url":"https:\/\/www.ebiohippo.com\/products\/mirna-purification-kit-bht15200024","provider":"BioHippo","version":"1.0","type":"link"}