{"product_id":"mitf-reporter-lentivirus-bhv19400217","title":"MITF Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMITF (microphthalmia-associated transcription factor) is a basic helix-loop-helix leucine zipper transcription factor and the master regulator of the melanocyte lineage. The melanocyte-specific isoform, MITF-M, controls melanocyte development, survival, and pigmentation by activating genes such as tyrosinase. MITF exists as multiple isoforms with cell-type-specific expression and is regulated at transcriptional, post-transcriptional, and post-translational levels by upstream signaling pathways including Wnt\/beta-catenin and BRAF\/ERK. In melanoma, MITF acts as a lineage-survival oncogene, and its activity influences the balance between proliferative and invasive cell states. These roles make MITF a central focus in pigmentation biology and melanoma research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe MITF Reporter Lentivirus is a transcription-factor reporter system for robust detection of MITF-M transcriptional activity in human and mouse cells. It uses multiple MITF response elements derived from the tyrosinase promoter to drive a fluorescent or luminescent reporter, with options including GFP, RFP, mCherry, BFP2, firefly luciferase, Gaussia luciferase, Renilla luciferase, and dual fluorescent-luciferase configurations. A constitutively expressed selection marker enables stable polyclonal cell line generation, with readout by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eApplications include studying melanocyte differentiation, MITF regulation by Wnt and MAPK signaling, and melanoma biology. Particles are purified by PEG precipitation and sucrose gradient centrifugation, making them effective in difficult-to-transfect primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the MITF transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251620471149,"sku":"LTV-0108-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362029363565,"sku":"LTV-0108-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362029396333,"sku":"LTV-0108-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362029429101,"sku":"LTV-0108-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362029461869,"sku":"LTV-0108-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362029494637,"sku":"LTV-0108-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/MITF-TAG-Puro-Fig1.jpg?v=1776934221","url":"https:\/\/www.ebiohippo.com\/products\/mitf-reporter-lentivirus-bhv19400217","provider":"BioHippo","version":"1.0","type":"link"}