{"product_id":"mmp1-human-hek-bhz11000110","title":"MMP1 Human, HEK","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch3 style=\"color:#1a5c58;margin:12px 0 4px\"\u003eProduct Description\u003c\/h3\u003e\n\u003cp\u003eMatrix Metalloproteinase-1 Human Recombinant, HEK\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch3 style=\"color:#1a5c58;margin:12px 0 4px\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003eMMP-1 (interstitial collagenase) can break down a wide range of substrates including types I, II, III, VII, VIII, and X collagens as well as L-Selectin, pro-TNF, IL-1?, IGFBP-3, IGFBP-5, casein, gelatin, ?1 antitrypsin, myelin basic protein, pro-MMP2 and pro-MMP9. A significant function of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling. MMP-1 is expressed in fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 can be divided into a number of distinct domains: a prodomain which is cleaved on activation, a catalytic domain containing the zinc binding site and a short hinge region with a carboxyl terminal domain. MMP1 is part of a cluster of MMP genes which localize to chromosome 11q22.3.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch3 style=\"color:#1a5c58;margin:12px 0 4px\"\u003eBiological Activity\u003c\/h3\u003e\n\u003cp\u003eThe activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is \u0026gt; 400 pmoles\/min\/µg.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP1 to 50µg\/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w\/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 2 hours.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch3 style=\"color:#1a5c58;margin:12px 0 4px\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width:100%;border-collapse:collapse;font-size:0.92em\"\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;padding:4px 8px;background:#e8f4f3;width:180px;font-weight:bold\"\u003ePurity\u003c\/th\u003e\n\u003ctd style=\"padding:4px 8px\"\u003eGreater than 95% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;padding:4px 8px;background:#e8f4f3;width:180px;font-weight:bold\"\u003eFormulation\u003c\/th\u003e\n\u003ctd style=\"padding:4px 8px\"\u003eThe MMP-1 is supplied as a 0.2µm filtered solution in MES, NaCl, Glycerol and Brij35.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;padding:4px 8px;background:#e8f4f3;width:180px;font-weight:bold\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"padding:4px 8px\"\u003eStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;padding:4px 8px;background:#e8f4f3;width:180px;font-weight:bold\"\u003eExpression System\u003c\/th\u003e\n\u003ctd style=\"padding:4px 8px\"\u003eHEK293 cells.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"2µg","offer_id":53340120121709,"sku":"ENZ-099-2µg","price":60.0,"currency_code":"USD","in_stock":true},{"title":"10µg","offer_id":53340175827309,"sku":"ENZ-099-10µg","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":53340175860077,"sku":"ENZ-099-1mg","price":4680.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-mmp-1-human-ENZ-099.png?v=1782158416","url":"https:\/\/www.ebiohippo.com\/products\/mmp1-human-hek-bhz11000110","provider":"BioHippo","version":"1.0","type":"link"}