{"product_id":"mmp3-human-hek-bhz11000491","title":"MMP3 Human, HEK","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch3 style=\"color:#1a5c58;margin:12px 0 4px\"\u003eProduct Description\u003c\/h3\u003e\n\u003cp\u003eMatrix Metalloproteinase-3 Human Recombinant, HEK\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch3 style=\"color:#1a5c58;margin:12px 0 4px\"\u003eOverview\u003c\/h3\u003e\n\u003cp\u003eMMP-3 enzyme is also known as Stromelysin-1or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. It dissolves the intervertebral nucleus pulposus and annulus fibrosus of Herniated Lumbar Intervertebral Disk . MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL1-beta.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch3 style=\"color:#1a5c58;margin:12px 0 4px\"\u003eBiological Activity\u003c\/h3\u003e\n\u003cp\u003eThe activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is \u0026gt; 150 pmoles\/min\/µg.Recombinant Human MMP-3 protein pro form needs to be activated with Chymotrypsin.Activation Protocol:1. Dilute MMP3 to 20µg\/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w\/v) and Brij 35, pH 7.5.2. Activate MMP3 by adding Chymotrypsin(Sigma, Catalog#C­3142,1mg\/ml stock in 1mM HCl) to a final concentration of 5ug\/ml.3. Incubate at 37°C for 30 minutes.4. Stop activation with 2mM PMSF. Pre-warm the PMSF to 37°C prior to adding to sample.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch3 style=\"color:#1a5c58;margin:12px 0 4px\"\u003eSpecifications\u003c\/h3\u003e\n\u003ctable style=\"width:100%;border-collapse:collapse;font-size:0.92em\"\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;padding:4px 8px;background:#e8f4f3;width:180px;font-weight:bold\"\u003ePurity\u003c\/th\u003e\n\u003ctd style=\"padding:4px 8px\"\u003eGreater than 95% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;padding:4px 8px;background:#e8f4f3;width:180px;font-weight:bold\"\u003eFormulation\u003c\/th\u003e\n\u003ctd style=\"padding:4px 8px\"\u003eThe MMP-3 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij35, pH 7.5.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;padding:4px 8px;background:#e8f4f3;width:180px;font-weight:bold\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"padding:4px 8px\"\u003eStore at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;padding:4px 8px;background:#e8f4f3;width:180px;font-weight:bold\"\u003eExpression System\u003c\/th\u003e\n\u003ctd style=\"padding:4px 8px\"\u003eHEK293 cells.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e","brand":"ProSpec-Tany TechnoGene Ltd","offers":[{"title":"2µg","offer_id":53340120187245,"sku":"ENZ-284-2µg","price":60.0,"currency_code":"USD","in_stock":true},{"title":"10µg","offer_id":53340176023917,"sku":"ENZ-284-10µg","price":145.0,"currency_code":"USD","in_stock":true},{"title":"1mg","offer_id":53340176056685,"sku":"ENZ-284-1mg","price":4680.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Prospecbio-mmp3-human-ENZ-284.png?v=1782158443","url":"https:\/\/www.ebiohippo.com\/products\/mmp3-human-hek-bhz11000491","provider":"BioHippo","version":"1.0","type":"link"}