{"product_id":"mouse-anti-dna-recombinant-autoantibody-pme77-bha21405131","title":"Mouse Anti-DNA Recombinant Autoantibody (PME77)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against DNA Recombinant Autoantibody (dsDNA) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eDNA is a molecular target commonly interrogated by antibody-based methods to measure protein expression, localization and regulation across biological systems. Antibodies against DNA support studies ranging from baseline expression profiling to stimulus- or disease-associated changes in abundance and subcellular distribution. For pathway-level interpretation, pair DNA detection with appropriate controls and complementary readouts (e.g., genetic perturbation or orthogonal assays).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eSubcellular localization is target- and context-dependent; use cell-type appropriate controls and, where possible, genetic KO\/KD as specificity controls.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eMolecular \u0026amp; Cellular Biology\u003c\/em\u003e research. Application suitability (e.g., ELISA, WB, IF) is indicated in the specifications; conditions should be optimized by titration.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, adjust permeabilization (e.g., mild detergent vs stronger conditions) based on expected localization; include secondary-only and isotype controls for background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of DNA Recombinant Autoantibody, and a positive control sample known to express DNA Recombinant Autoantibody.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036917031277,"sku":"YP373073-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037506986349,"sku":"YP373073-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037507019117,"sku":"YP373073-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/YP373073-SDS-PAGE-1.jpg?v=1771745757","url":"https:\/\/www.ebiohippo.com\/products\/mouse-anti-dna-recombinant-autoantibody-pme77-bha21405131","provider":"BioHippo","version":"1.0","type":"link"}