{"product_id":"mouse-anti-human-igg-lambda-chain-monoclonal-antibody-fitc-bha21416408","title":"Mouse Anti-Human IgG, Lambda Chain Monoclonal Antibody, FITC","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nMouse Anti-Human IgG, Lambda Chain Monoclonal Antibody, FITC is intended for detection of \u003cstrong\u003eHuman IgG, Lambda Chain\u003c\/strong\u003e. Commonly used in ELISA, IF, WB applications. Typical workflows include Select staining controls in cells\/tissues (isotype\/KD\/KO), Validate antibody specificity by WB with KD\/KO control lysates, Detect target localization by IF\/ICC in cultured cells, Phenotype target-positive cells by flow cytometry in PBMCs\/cell suspensions, Develop ELISA pairing using capture\/detection antibodies and a standard curve, Build multiplex staining panel using conjugated antibody and controls. Relevant research areas: Molecular \u0026amp; Cellular Biology.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Lambda Chain Monoclonal Antibody, Mouse Anti-Human IgG, Second antibody, Second Ab, FITC.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent details:\u003c\/strong\u003e Host: Mouse; Clonality: Monoclonal; Isotype: IgG2a; Conjugate: FITC; Purification: Protein A\/G purified from cell culture supernatant.; Format: Liquid; Reactivity: Human.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eHuman IgG, Lambda Chain (Lambda Chain Monoclonal Antibody) is the antigen described for this antibody and is commonly evaluated for expression and localization in relevant model systems. It is frequently investigated in contexts related to Molecular \u0026amp; Cellular Biology. Common alternative names and symbols used in the literature include: Mouse Anti-Human IgG, Second antibody, Second Ab, FITC. Based on the annotated name\/class, the antigen is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this reagent helps\u003c\/h3\u003e\n\u003cp\u003eThis antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.\u003c\/p\u003e\n\n\u003ch3\u003eAntigen \/ immunogen context\u003c\/h3\u003e\u003cp\u003eNature Protein\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody\/reagent to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eInclude appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives\/negatives).\u003c\/li\u003e\n\u003cli\u003eFor flow cytometry, include Fc blocking when applicable, use a viability dye, and consider staining live cells for surface markers prior to fixation\/permeabilization.\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, test fixation (PFA vs methanol) and permeabilization conditions to preserve epitope accessibility while minimizing background.\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, include fresh protease inhibitors (and phosphatase inhibitors for PTM targets) and verify the dominant band at the expected size; additional bands may reflect isoforms, processing, or cross-reactivity.\u003c\/li\u003e\n\u003cli\u003eFor immunoprecipitation, use gentle lysis conditions, maintain cold temperatures, and validate enrichment relative to an isotype control.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize capture\/detection pairing and run a standard curve in matrix-matched diluent when possible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage\u003c\/h3\u003e\u003cp\u003eStore at 4°C 12 months. Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.\u003c\/p\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037202211181,"sku":"HP678415-50UG","price":206.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037840597357,"sku":"HP678415-100UG","price":338.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/mouse-anti-human-igg-lambda-chain-monoclonal-antibody-fitc-bha21416408","provider":"BioHippo","version":"1.0","type":"link"}