{"product_id":"mouse-anti-kcnn4-kca3-1-sk4-extracellular-pe-antibody-bha21300574","title":"Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-PE Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eMouse Anti-KCNN4 (KCa3.1, SK4) (extracellular)-PE Antibody is an antibody targeting IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel Monoclonal raised in Mouse (R-Phycoerythrin (R-PE)). This antibody is commonly used in IC, IF, IFC, IHC, LCI, WB to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel (also reported as IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e Extracellular loop.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Recognizes KCNN4 from human, rat, and mouse samples (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Human, Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis (unlabeled antibody), and direct flow cytometry (labeled antibody)..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eConjugate\/format:\u003c\/strong\u003e R-Phycoerythrin (R-PE) (may affect detection channel and background).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody type:\u003c\/strong\u003e Monoclonal \/ IgM.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eKCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eLinking transporter\/channel abundance to ionic homeostasis and excitability-related phenotypes.\u003c\/li\u003e   \u003cli\u003eStudying compartment-specific localization (surface vs intracellular pools) and trafficking dynamics.\u003c\/li\u003e   \u003cli\u003eCombining antibody readouts with functional assays for more complete interpretation.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare target abundance\/size across lysates and conditions; consider isoforms\/PTMs.\u003c\/li\u003e   \u003cli\u003eImmunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.\u003c\/li\u003e   \u003cli\u003eImmunofluorescence\/ICC: assess subcellular localization and co-localization with markers in cells or sections.\u003c\/li\u003e   \u003cli\u003eFlow cytometry (direct\/indirect): quantify target-positive populations and shifts in expression across subsets.\u003c\/li\u003e   \u003cli\u003eLive cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"50 mcl \/ 1","offer_id":53064835039597,"sku":"ALM-051-PE-50MCL-1","price":797.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 2","offer_id":53064912404845,"sku":"ALM-051-PE-50MCL-2","price":1397.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 5","offer_id":53064912437613,"sku":"ALM-051-PE-50MCL-5","price":2743.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 10","offer_id":53064912470381,"sku":"ALM-051-PE-50MCL-10","price":3764.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ALM-051-PE_J774_2.5mic_FC2.png?v=1772460997","url":"https:\/\/www.ebiohippo.com\/products\/mouse-anti-kcnn4-kca3-1-sk4-extracellular-pe-antibody-bha21300574","provider":"BioHippo","version":"1.0","type":"link"}