{"product_id":"mouse-anti-rat-p75-ngf-receptor-extracellular-antibody-bha21300635","title":"Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eMouse Anti-Rat p75 NGF Receptor (extracellular) Antibody is an antibody targeting p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271 Monoclonal raised in Mouse (Unconjugated). This antibody is commonly used in IF, IFC, IHC, WB to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271 (also reported as p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Recognizes p75NTR from rat samples only (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Rat.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecificity statement (as provided):\u003c\/strong\u003e 192-IgG reacts with rat p75NTR. It does not cross-react with p75NTR of human or mouse origin..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eConjugate\/format:\u003c\/strong\u003e Unconjugated (may affect detection channel and background).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody type:\u003c\/strong\u003e Monoclonal \/ IgG1.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003ep75NTR known as a cell surface receptor for NGF, BDNF, NT-3 and NT-4, is also a receptor for b-amyloid, and for the myelin axon repellant protein MAG.1,2,4,6 Binding of neurotrophins induces receptor dimerization followed by phosphorylation of receptor kinase residues and recruitment of intracellular proteins involved in signal transduction. p75NTR functions alone, or in functional complexes with the neurotrophin receptors trkA, trkB and trkC. p75NTR signals via interaction with different transducers such as TRAF6, IRAK, and RIP-2, activating NF-κB, JNK and p38, and inactivating rhoA.1,3,6 Receptor degradation is mediated by a metalloproteinase-dependent shedding of the extracellular domain.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eProfiling immune-marker expression across cell subsets with single-cell or flow-based readouts.\u003c\/li\u003e   \u003cli\u003eConnecting receptor\/ligand levels to activation state and cytokine programs.\u003c\/li\u003e   \u003cli\u003eApplying genetic perturbation or orthogonal assays to support specificity and interpretation.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare target abundance\/size across lysates and conditions; consider isoforms\/PTMs.\u003c\/li\u003e   \u003cli\u003eImmunohistochemistry (IHC): examine spatial distribution in tissue and relate signal to cell-type composition.\u003c\/li\u003e   \u003cli\u003eImmunofluorescence\/ICC: assess subcellular localization and co-localization with markers in cells or sections.\u003c\/li\u003e   \u003cli\u003eFlow cytometry (direct\/indirect): quantify target-positive populations and shifts in expression across subsets.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"10 mcg \/ 1","offer_id":53064836415853,"sku":"AN-170-10MCG-1","price":377.0,"currency_code":"USD","in_stock":true},{"title":"100 mcg \/ 1","offer_id":53064918008173,"sku":"AN-170-100MCG-1","price":697.0,"currency_code":"USD","in_stock":true},{"title":"250 mcg \/ 1","offer_id":53064918040941,"sku":"AN-170-250MCG-1","price":977.0,"currency_code":"USD","in_stock":true},{"title":"50 mcg \/ 1","offer_id":53064918073709,"sku":"AN-170-50MCG-1","price":797.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/AN-170-APR-082_IH.jpg?v=1772461008","url":"https:\/\/www.ebiohippo.com\/products\/mouse-anti-rat-p75-ngf-receptor-extracellular-antibody-bha21300635","provider":"BioHippo","version":"1.0","type":"link"}