{"product_id":"mouse-astrocytes-spinal-cord-ma-sc-bhc18500282","title":"Mouse Astrocytes-spinal cord (MA-sc)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eMouse Astrocytes-spinal cord (MA-sc)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Mouse Astrocytes derived from Spinal Cord (spinal cord) within the Nervous system.\u003c\/p\u003e\n\u003cp\u003eAstrocytes are the major cell type in the mammalian brain. They have been implicated in a variety of supportive functions for their partner neurons in the CNS, such as neuronal guidance during development, ion and water homeostasis, blood flow regulation, neurotransmission, energy metabolism, and immune defense [1]. Recent studies have shown that spinal cord astrocytes contribute to neuroinflammation by chemokine expression which leads to the recruitment of “inflammatory” monocytes and neutrophils to the lesion site [2]. Experimentally, spinal cord astrocytes have also been used to study the wobbler mutation and muscular dystrophy in mice [3] and astrogliosis [4]. As the recognition of the importance of astrocytes in nervous system functioning is increasing, specifically regarding the modulation of neural activity, astrocyte cultures are continuing to provide a useful tool in exploring the diverse properties and functions of these cells. iXCells Biotechnologies provides high quality Mouse Astrocytes-spinal cord (MA-sc), which are isolated from neonate day two mouse spinal cord and cryopreserved at P1, with \u0026gt;0.5 million cells in each vial. MA-sc express GFAP are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They can further expand for 5 population doublings in Astrocyte Medium (Cat# MD-0039) under the condition suggested by iXCells Biotechnologies.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Astrocytes (Primary Cells, Custom Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Spinal Cord; spinal cord; Nervous\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-1 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eNeural and glial cell models support studies of neuronal signaling, synaptic biology, neuroinflammation, and cell-type–specific responses to injury or disease-relevant stimuli.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n  \u003cli\u003eGrowth of human-relevant neural models (including glial components) to study circuit- and inflammation-linked phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify neurite outgrowth and synaptic marker profiles in neural cultures\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eMeasure neuroinflammatory signaling in neuron–glia or microglia-enriched models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500282\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ Inquire about custom lot sizes, package sizes and marker characterization","offer_id":53197817545069,"sku":"10MU-012","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10MU-012.webp?v=1782157704","url":"https:\/\/www.ebiohippo.com\/products\/mouse-astrocytes-spinal-cord-ma-sc-bhc18500282","provider":"BioHippo","version":"1.0","type":"link"}