{"product_id":"mouse-bone-marrow-derived-macrophage-mbmdm-bhc18500091","title":"Mouse Bone Marrow Derived Macrophage (mBMDM)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eMouse Bone Marrow Derived Macrophage (mBMDM)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Mouse Immune Cells AND Hematopoietic Cells derived from Bone Marrow (Bone Marrow Derived Macrophage) within the Blood system.\u003c\/p\u003e\n\u003cp\u003eMacrophages are a type of white blood cell differentiated from circulating bone marrow-derived monocytes. Macrophages are responsible for detecting, engulfing and digesting cellular debris, apoptotic cells, and invading pathogens in a process called phagocytosis. Macrophages can be identified by several specific cell surface proteins including CD11b, CD14, F4\/80 (mice)\/EMR1 (human), MAC-1\/MAC-3, and CD68 by immunohistochemistry or flow cytometry analysis [1] . T Bone marrow-derived macrophages are suitable for numerous applications including phagocytosis, gene expression profiling, and Morphological examination of cytospins using histological stains (e.g., May-Grünwald-Giemsa staining), etc. [2] . iXCells Biotechnologies provides Mouse Bone Marrow Derived Macrophage (mBMDM), which were differentiated in the presence of M-CSF using the bone marrow cells isolated from adult C57BL\/6 mice (Figure 1). mBMDM are harvested at P0 and delivered freshly or frozen. For frozen cells, each vial contains ≥ 5 million cells in 1 mL volume. mBMDMs are characterized by flow cytometry analysis with \u0026gt;97% CD11b+ purity (Figure 2). mBMDMs are negative for mycoplasma, bacteria, yeast, and fungi. It is recommended to use Macrophage Culture Medium (Cat # MD-0097) for in vitro culturing of mBMDM. However, mBMDMs are not recommended for long-term cultures or further expansion. Figure 1. Mouse Bone Marrow Derived Macrophage (mBMDM). (A) Phase contrast images. (B) The purity of the mBMDM was measured using CD11b+ antibody by flow cytometry analysis.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Immune Cells AND Hematopoietic Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Bone Marrow; Bone Marrow Derived Macrophage; Blood\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDonor background:\u003c\/strong\u003e Age: Adult\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-1 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCells originating from the Blood system are commonly studied to understand tissue-specific physiology, signaling, and responses to perturbations in controlled in vitro settings.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n  \u003cli\u003eExpansion of high-dimensional immune phenotyping and perturbation screens to map activation states and functional programs.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eStimulate immune cells and quantify activation markers and cytokine release\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003ePerform immune profiling by multiparameter flow cytometry to resolve major subsets\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500091\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 5 million cells\/vial","offer_id":53197814792557,"sku":"10MU-030","price":550.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10MU-030.webp?v=1782157703","url":"https:\/\/www.ebiohippo.com\/products\/mouse-bone-marrow-derived-macrophage-mbmdm-bhc18500091","provider":"BioHippo","version":"1.0","type":"link"}