{"product_id":"mouse-crp-c-reactive-protein-microsample-elisa-kit-bhe15212680","title":"Mouse CRP(C Reactive Protein) Microsample ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eCRP(C Reactive Protein) Microsample\u003c\/strong\u003e is an acute-phase inflammatory marker that can rise during systemic inflammation, infection, or tissue injury.\u003c\/p\u003e\u003cp\u003eAcute-phase proteins are often used to monitor overall inflammatory burden and compare disease severity across cohorts.\u003c\/p\u003e\u003cp\u003eBecause they integrate signals from multiple inflammatory pathways, they are useful for screening and for tracking response-to-treatment trends over time.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eCRP(C Reactive Protein) Microsample\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eMouse\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CRP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 1.56-100 µg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 1.09 µg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966442795373,"sku":"ELK10186MS-96T","price":488.8,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966442828141,"sku":"ELK10186MS-48T","price":344.5,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966442860909,"sku":"ELK10186MS-96TX5","price":2077.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_4e89b961-c2ff-40de-b6ca-3b364fb67c8e.jpg?v=1771848710","url":"https:\/\/www.ebiohippo.com\/products\/mouse-crp-c-reactive-protein-microsample-elisa-kit-bhe15212680","provider":"BioHippo","version":"1.0","type":"link"}