{"product_id":"mouse-eg-vegf-endocrine-gland-derived-vascular-endothelial-growth-factor-elisa-kit-bhe15206949","title":"Mouse EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eEG-VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor)\u003c\/strong\u003e is a growth factor–related marker involved in cellular proliferation, differentiation, or tissue remodeling processes.\u003c\/p\u003e\u003cp\u003eGrowth factor concentrations can reflect changes in tissue repair, fibrosis, angiogenesis, or tumor microenvironment signaling depending on the specific target and model.\u003c\/p\u003e\u003cp\u003eBecause many growth factors act locally and are regulated post-transcriptionally, protein quantification can add clarity beyond mRNA-only measurements.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eEG-VEGF (Endocrine Gland Derived Vascular Endothelial Growth Factor)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eMouse\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 15.63-1000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 5.8 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52965696930157,"sku":"ELK2479-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52965696962925,"sku":"ELK2479-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52965696995693,"sku":"ELK2479-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_993a9dbd-e2e7-45d2-804b-7cfe96865754.jpg?v=1771844844","url":"https:\/\/www.ebiohippo.com\/products\/mouse-eg-vegf-endocrine-gland-derived-vascular-endothelial-growth-factor-elisa-kit-bhe15206949","provider":"BioHippo","version":"1.0","type":"link"}