{"product_id":"mouse-igg2a-l234a-l235a-p329g-kappa-recombinant-isotype-control-antibody-hyhel-10-bha21421250","title":"Mouse IgG2a (L234A\/L235A\/P329G), kappa Recombinant Isotype Control Antibody (HyHEL-10)","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eIsotype Control\u003c\/span\u003e\u003cp\u003eMouse IgG2a (L234A\/L235A\/P329G), kappa Recombinant Isotype Control Antibody (HyHEL-10) is a monoclonal isotype control antibody (clone HyHEL-10). Isotype control antibodies match the host species, isotype and conjugate of a test antibody but are directed against an antigen absent from the sample, providing a measure of non-specific background binding (Fc-receptor interactions, non-specific membrane adhesion) in immunoassays.\u003c\/p\u003e\n\u003cp\u003eUse this control alongside mouse-derived IgG2a (L234A\/L235A\/P329G) kappa primary antibodies to set gating and background thresholds.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG2a (L234A\/L235A\/P329G), kappa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003eMonoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClone Number\u003c\/th\u003e\n\u003ctd\u003eHyHEL-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003eProtein A\/G purified from cell culture supernatant.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Buffer\u003c\/th\u003e\n\u003ctd\u003e0.01M PBS, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin Level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;10 EU\/mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications \u0026amp; Recommended Use\u003c\/h2\u003e\n\u003cp\u003eVendor-listed applications: \u003cstrong\u003eFlow Cytometry\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003eRun the isotype control at the same concentration and staining conditions as the corresponding test antibody to obtain a matched background reference.\u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.\u003c\/p\u003e\n\u003cp\u003eSupplied in 0.01M PBS, pH 7.4.\u003c\/p\u003e\n\u003ch2\u003eSafety\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for diagnostic or therapeutic procedures. Follow standard laboratory safety practices when handling.\u003c\/p\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"50 ug","offer_id":53820265398637,"sku":"MF533017-50UG","price":311.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53820267168109,"sku":"MF533017-100UG","price":411.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53820267200877,"sku":"MF533017-1MG","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/MF533017-SDS-PAGE-1.jpg?v=1787867099","url":"https:\/\/www.ebiohippo.com\/products\/mouse-igg2a-l234a-l235a-p329g-kappa-recombinant-isotype-control-antibody-hyhel-10-bha21421250","provider":"BioHippo","version":"1.0","type":"link"}