{"product_id":"mouse-insl5-insulin-like-protein-5-microsample-elisa-kit-bhe15211206","title":"Mouse INSL5(Insulin Like Protein 5) Microsample ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eINSL5(Insulin Like Protein 5) Microsample\u003c\/strong\u003e is a metabolic hormone\/mediator that influences energy balance, glucose\/lipid metabolism, or endocrine signaling.\u003c\/p\u003e\u003cp\u003eEndocrine marker levels can help connect physiological phenotypes (diet, obesity, diabetes models) with molecular pathway changes.\u003c\/p\u003e\u003cp\u003eQuantitative profiling supports group comparisons and can be paired with metabolic readouts such as glucose tolerance, insulin sensitivity, or lipid panels.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eINSL5(Insulin Like Protein 5) Microsample\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eMouse\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse INSL5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse INSL5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse INSL5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse INSL5 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 15.63-1000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 6.2 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966277710189,"sku":"ELK6081MS-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966277742957,"sku":"ELK6081MS-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966277775725,"sku":"ELK6081MS-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_87bb644e-119e-45ad-9444-19c74fb245e9.jpg?v=1771847602","url":"https:\/\/www.ebiohippo.com\/products\/mouse-insl5-insulin-like-protein-5-microsample-elisa-kit-bhe15211206","provider":"BioHippo","version":"1.0","type":"link"}