{"product_id":"mouse-myc-shrna-lentivirus-bhv19400165","title":"Mouse Myc shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMyc (c-Myc) is a basic helix-loop-helix leucine zipper transcription factor and a classic proto-oncogene. Heterodimerizing with Max, Myc binds E-box elements to control a broad transcriptional program governing cell growth, proliferation, ribosome biogenesis, metabolism, and apoptosis. In normal cells Myc expression is tightly coupled to mitogenic signaling, but amplification, translocation, or protein stabilization can produce constitutive activity that drives uncontrolled proliferation. Myc deregulation is a hallmark of many tumors, and the gene is widely used in mouse models of cancer. As a master regulator of proliferation, Myc is a central target for loss-of-function studies of tumor cell growth and survival.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Mouse Myc shRNA Lentivirus delivers high-titer shRNA lentiviral particles that specifically silence the mouse Myc proto-oncogene. The shRNA is expressed from a third-generation, self-inactivating lentiviral backbone and has been validated to achieve at least 70% knockdown using a rapid fluorescence-based assay rather than qPCR. Co-expressed fluorescent reporters (GFP or RFP, with optional luciferase) and antibiotic selection markers (puromycin or blasticidin) allow tracking of transduced cells and rapid establishment of stable knockdown lines. The shRNA lentivirus set provides particles produced from a mix of two independent validated shRNAs plus a matched scrambled-shRNA control. Ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells, for loss-of-function studies of Myc.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting Myc (NCBI Accession: NM_001177352.1) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251620733293,"sku":"LSV-0049-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311266652525,"sku":"LSV-0049-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311266685293,"sku":"LSV-0049-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311266718061,"sku":"LSV-0049-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311266750829,"sku":"LSV-0049-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311266783597,"sku":"LSV-0049-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311266816365,"sku":"LSV-0049-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311266849133,"sku":"LSV-0049-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0049-mMyc-sh2-Fig1.jpg?v=1776934144","url":"https:\/\/www.ebiohippo.com\/products\/mouse-myc-shrna-lentivirus-bhv19400165","provider":"BioHippo","version":"1.0","type":"link"}