{"product_id":"mouse-pdl1-shrna-lentivirus-bhv19400159","title":"Mouse PDL1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003ePD-L1 (programmed death-ligand 1, also called B7-H1, encoded by Cd274) is a transmembrane immune checkpoint ligand expressed on tumor cells, antigen-presenting cells, and other tissues. By engaging the inhibitory receptor PD-1 on activated T cells, PD-L1 delivers a suppressive signal that limits T cell proliferation, cytokine production, and cytotoxic activity, contributing to peripheral tolerance and prevention of excessive immune responses. Tumors frequently upregulate PD-L1, often in response to interferon-gamma, to evade immune surveillance. The PD-1\/PD-L1 axis is a central target of checkpoint-blockade immunotherapy, making PD-L1 an important focus of cancer immunology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Mouse PD-L1 shRNA Lentivirus delivers validated short hairpin RNA targeting mouse Cd274 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% PD-L1 knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of immune checkpoint signaling, tumor immune evasion, and checkpoint-blockade research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting Cd274 (NCBI Accession: NM_021893.3) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251616342381,"sku":"LSV-0043-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311253840237,"sku":"LSV-0043-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311253873005,"sku":"LSV-0043-5-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311253905773,"sku":"LSV-0043-5-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311253938541,"sku":"LSV-0043-5-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311253971309,"sku":"LSV-0043-5-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311254004077,"sku":"LSV-0043-5-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311254036845,"sku":"LSV-0043-5-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311254069613,"sku":"LSV-0043-5-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311254102381,"sku":"LSV-0043-5-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0043-m-Pdl1-sh-fig1.jpg?v=1776934150","url":"https:\/\/www.ebiohippo.com\/products\/mouse-pdl1-shrna-lentivirus-bhv19400159","provider":"BioHippo","version":"1.0","type":"link"}