{"product_id":"mouse-phospho-insulin-receptor-subunit-ptyr1162-1163-elisa-kit-bhe15208507","title":"Mouse phospho-Insulin Receptor, ? Subunit(pTyr1162\/1163) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003ephospho-Insulin Receptor, β Subunit (pTyr1162\/1163)\u003c\/strong\u003e is a metabolic hormone\/mediator that influences energy balance, glucose\/lipid metabolism, or endocrine signaling.\u003c\/p\u003e\u003cp\u003eEndocrine marker levels can help connect physiological phenotypes (diet, obesity, diabetes models) with molecular pathway changes.\u003c\/p\u003e\u003cp\u003eQuantitative profiling supports group comparisons and can be paired with metabolic readouts such as glucose tolerance, insulin sensitivity, or lipid panels.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003ephospho-Insulin Receptor, β Subunit (pTyr1162\/1163)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eMouse\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IR (Phospho) [pY1162\/1163]. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IR (Phospho) [pY1162\/1163]. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IR (Phospho) [pY1162\/1163], biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IR (Phospho) [pY1162\/1163] in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 1.57-100 U\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.68 U\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52965967790445,"sku":"ELK0766-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52965967823213,"sku":"ELK0766-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52965967855981,"sku":"ELK0766-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_d9b736a7-a742-41db-a707-368ee3baa0a0.jpg?v=1771845599","url":"https:\/\/www.ebiohippo.com\/products\/mouse-phospho-insulin-receptor-subunit-ptyr1162-1163-elisa-kit-bhe15208507","provider":"BioHippo","version":"1.0","type":"link"}