{"product_id":"mouse-pkc-1-protein-kinase-c-beta-1-microsample-elisa-kit-bhe15212399","title":"Mouse PKC?1(Protein Kinase C Beta 1) Microsample ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003ePKCβ1(Protein Kinase C Beta 1) Microsample\u003c\/strong\u003e is associated with intracellular signaling regulation, often within phosphorylation-dependent pathways that control activation, proliferation, or differentiation.\u003c\/p\u003e\u003cp\u003eWhile kinase activity is frequently assessed by phospho-readouts, total protein abundance can also shift with pathway rewiring, feedback, or changes in cell composition.\u003c\/p\u003e\u003cp\u003eProtein quantification can complement phospho-specific assays and help interpret whether signaling changes are driven by abundance, activation state, or both.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003ePKCβ1(Protein Kinase C Beta 1) Microsample\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eMouse\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PKCβ1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PKCβ1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PKCβ1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PKCβ1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates, cell lysates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 0.32-20 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.17 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966414844269,"sku":"ELK0485MS-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966414877037,"sku":"ELK0485MS-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966414909805,"sku":"ELK0485MS-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_fd797e3d-c93e-41e7-bb45-49c6e4291586.jpg?v=1771848682","url":"https:\/\/www.ebiohippo.com\/products\/mouse-pkc-1-protein-kinase-c-beta-1-microsample-elisa-kit-bhe15212399","provider":"BioHippo","version":"1.0","type":"link"}