{"product_id":"mouse-primary-hepatic-sinusoidal-endothelial-cells-bhc10901867","title":"Mouse Primary Hepatic Sinusoidal Endothelial Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMouse Primary Hepatic Sinusoidal Endothelial Cells (HSECs) are specialized endothelial cells isolated from mouse liver, offering a physiologically relevant model for studying liver microvasculature, immune interactions, and drug metabolism. These cells maintain key fenestration and functional characteristics in vitro, making them ideal for research in liver biology, disease modeling, and toxicity testing. The product is supplied as frozen cells.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Mouse (M. musculus)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Liver\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding guidance:\u003c\/strong\u003e 5,000 - 10,000 cells\/cm²\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) Endothelial Cell Medium Kit (TM154) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eEndothelial cells line the luminal surface of blood vessels and regulate barrier properties, hemostatic balance, immune cell trafficking, angiogenic signaling, and tissue-specific exchange processes.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary endothelial models are used to study barrier integrity, inflammatory activation, and permeability changes in tissue-specific vascular beds.\u003c\/li\u003e\n\u003cli\u003eCo-culture systems with immune, stromal, or parenchymal cells are increasingly used to model microenvironment-dependent signaling.\u003c\/li\u003e\n\u003cli\u003eResearchers often compare donor, vessel-bed, or organ-specific phenotypes to capture biologically relevant heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAssess barrier function, adhesion molecule regulation, or cytokine-driven endothelial activation in vitro.\u003c\/li\u003e\n\u003cli\u003eModel angiogenic responses, matrix interactions, and vessel-bed-specific signaling under defined culture conditions.\u003c\/li\u003e\n\u003cli\u003eUse in co-culture or transwell systems to study leukocyte transmigration and paracrine crosstalk.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) Endothelial Cell Medium Kit (TM154) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 5,000 - 10,000\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10901867\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195752898925,"sku":"T4600","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/c39SR78Xx7RA3aau7ouIGMA7Lgken9dTxfi5ClF0.png?v=1775322480","url":"https:\/\/www.ebiohippo.com\/products\/mouse-primary-hepatic-sinusoidal-endothelial-cells-bhc10901867","provider":"BioHippo","version":"1.0","type":"link"}