{"product_id":"mouse-pro-mmp-9-pro-matrix-metalloproteinase-9-microsample-elisa-kit-bhe15210690","title":"Mouse Pro-MMP-9(Pro-Matrix Metalloproteinase 9) Microsample ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003ePro-MMP-9(Pro-Matrix Metalloproteinase 9) Microsample\u003c\/strong\u003e is a matrix metalloproteinase-related enzyme marker linked to extracellular matrix (ECM) remodeling.\u003c\/p\u003e\u003cp\u003eMMP levels are frequently tracked in studies of inflammation, fibrosis, wound healing, and cancer invasion\/metastasis where ECM turnover is important.\u003c\/p\u003e\u003cp\u003eBecause MMP regulation can involve secretion, activation, and inhibition, protein quantification supports a fuller interpretation alongside functional assays.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003ePro-MMP-9(Pro-Matrix Metalloproteinase 9) Microsample\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eMouse\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-MMP-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Pro-MMP-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Pro-MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Pro-MMP-9 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, tissue homogenates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 0.79-50 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.478 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966225379693,"sku":"ELK8500MS-96T","price":764.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966225412461,"sku":"ELK8500MS-48T","price":535.6,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966225445229,"sku":"ELK8500MS-96TX5","price":3248.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_2ea2fa56-ada3-4b5e-b20e-bd4b6c6f937d.jpg?v=1771846844","url":"https:\/\/www.ebiohippo.com\/products\/mouse-pro-mmp-9-pro-matrix-metalloproteinase-9-microsample-elisa-kit-bhe15210690","provider":"BioHippo","version":"1.0","type":"link"}