{"product_id":"mouse-skeletal-muscle-myoblasts-mskmm-bhc18500093","title":"Mouse Skeletal Muscle Myoblasts (MSkMM)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eMouse Skeletal Muscle Myoblasts (MSkMM)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Mouse Myoblasts derived from Skeletal Muscle (Skeletal Muscle) within the Musculoskeletal system.\u003c\/p\u003e\n\u003cp\u003eMyoblast is a type of progenitor cells that differentiates to give rise to muscle cells [1] . They are the main effector cells in the myogenesis by fusing with each other to injured myofibers leading to the formation of new myofibers or regenerations of skeletal muscle in adults [2] . Many steps of myogenesis can be recapitulated through in vitro differentiation of myoblasts into myotubes. Primary myoblasts have been suggested as the most physiologically relevant model for studying myogenesis in vitro [3] . MSkMM from iXCells Biotechnologies are derived from mouse skeletal satellite cells isolated from mouse gastrocnemius (GA) and tibialis anterior (TA) muscles of both hind limbs by culturing them on matrigel-coated plastic dishes (Figure 1). MSkMM are cryopreserved at passage one and delivered frozen. Each vial contains ≥5 x 10 5 cells\/vial. MSkMM are characterized by immunofluorescence with antibodies specific to MyoD and Desmin (Figure 2). MSkMM can be in vitro differentiated into myotube using Mouse Skeletal Muscle Myoblast Differentiation Medium ( Cat#MD-0065 ). MSkMM are guaranteed to further expand two passages using the culture medium provided by iXCells. These cells are negative for mycoplasma, bacteria, yeast and fungi. Figure 1. Mouse Skeletal Muscle Myoblasts (MSkMM) were recovered and seeded at 1X10 4 \/cm 2 in Mouse Skeletal Muscle Myoblast Growth Medium (Cat# MD-0064). Phase contrast images were taken at the indicated time post recovery.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Myoblasts (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Skeletal Muscle; Skeletal Muscle; Musculoskeletal\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-1 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCells originating from the Musculoskeletal system are commonly studied to understand tissue-specific physiology, signaling, and responses to perturbations in controlled in vitro settings.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eEvaluate matrix remodeling and differentiation programs in musculoskeletal cell models\u003c\/li\u003e\n  \u003cli\u003eScreen compounds or genetic perturbations for phenotype modulation using viability or imaging endpoints\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500093\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 0.5 million cells\/vial","offer_id":53197815218541,"sku":"10MU-033","price":929.8,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10MU-033.png?v=1775378642","url":"https:\/\/www.ebiohippo.com\/products\/mouse-skeletal-muscle-myoblasts-mskmm-bhc18500093","provider":"BioHippo","version":"1.0","type":"link"}