{"product_id":"mouse-tissue-direct-pcr-kit-bht15200029","title":"Mouse Tissue (Mouse Tail, Mouse Ear) Direct PCR Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ePCR Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis kit is specially designed for mouse transgene identification and genotyping. It can directly and rapidly extract genomic DNA from mouse tissue (such as mouse tail, mouse ear, mouse toe, muscle, etc.) for subsequent PCR amplification and detection. The entire process does not require homogenization, disruption, overnight digestion, phenol-chloroform extraction, DNA precipitation or column purification. The sample requirement is small: 5–10 mg of mouse tissue or 1–5 mm of mouse tail is enough for genotyping.\u003c\/p\u003e\n\u003cp\u003eThe 2× Direct PCR Mix provided by this kit is a PCR master mix with high amplification compatibility, which can perform efficient and specific amplification without completely removing impurities such as proteins. The master mix contains antibody-modified Hot Star Taq DNA Polymerase, dNTPs, Mg²⁺ and other components, and only needs to add template and primers. The 2× Direct PCR Mix is pre-mixed with electrophoresis dyes, which can be directly detected by electrophoresis after the reaction is completed. The 3' end of the PCR product has an A, which can be used for TA cloning. Tissue is lysed at 55°C for 30 min and centrifuged at 12,000 rpm for 2 min before PCR; the recommended cycling is 94°C for 1–5 min, then 35 cycles of 94°C for 30 s, 60°C for 30 s and 72°C for 30 s\/kb, with a final 72°C for 10 min.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eDirect PCR kit: Tissue Lysis Buffer, Digestive Enzyme, 2× Direct PCR Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003eMouse tissue such as tail, ear, toe and muscle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e5–10 mg mouse tissue or 1–5 mm mouse tail\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePolymerase\u003c\/th\u003e\n\u003ctd\u003eAntibody-modified Hot Star Taq DNA Polymerase (hot start)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTracking dye\u003c\/th\u003e\n\u003ctd\u003eElectrophoresis dyes premixed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction volume\u003c\/th\u003e\n\u003ctd\u003e20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input\u003c\/th\u003e\n\u003ctd\u003e1–5% of the reaction volume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAmplicon length\u003c\/th\u003e\n\u003ctd\u003eWithin 2 kb recommended\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct ends\u003c\/th\u003e\n\u003ctd\u003e3' A overhang, suitable for TA cloning\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTissue Lysis Buffer\u003c\/td\u003e\n\u003ctd\u003e1.25 mL × 4\u003c\/td\u003e\n\u003ctd\u003e20 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestive Enzyme\u003c\/td\u003e\n\u003ctd\u003e250 µL\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2× Direct PCR Mix\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003ctd\u003e1 mL × 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eddH₂O\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e4 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMouse transgene identification\u003c\/li\u003e\n\u003cli\u003eMouse genotyping from tail, ear and toe tissue\u003c\/li\u003e\n\u003cli\u003eDirect PCR from mouse tissue without DNA purification\u003c\/li\u003e\n\u003cli\u003eTA cloning of PCR products\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSimple and fast: suitable for one-step gene identification from mouse tail, mouse ear, mouse toe and other tissues.\u003c\/li\u003e\n\u003cli\u003eHigh specificity: This product uses antibody-modified hot-start Taq enzyme, which has high template affinity and amplification specificity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components are stored at -20°C for 3 years.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid cross-contamination between samples.\u003c\/li\u003e\n\u003cli\u003eUse fresh tissue.\u003c\/li\u003e\n\u003cli\u003eMinimize freeze-thaw cycles.\u003c\/li\u003e\n\u003cli\u003eEstablish negative and positive controls for each run.\u003c\/li\u003e\n\u003cli\u003eKeep the PCR product length within 2 kb for optimal efficiency.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP033\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200029\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"200 T","offer_id":53965512474989,"sku":"EP033-200T","price":260.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965515325805,"sku":"EP033-50TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965515358573,"sku":"EP033-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP033-a-1.jpg?v=1789419326","url":"https:\/\/www.ebiohippo.com\/products\/mouse-tissue-direct-pcr-kit-bht15200029","provider":"BioHippo","version":"1.0","type":"link"}