{"product_id":"mouse-tissue-direct-pcr-kit-pro-long-pcr-bht20800005","title":"Mouse Tissue Direct PCR Kit Pro (Long PCR)","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp align=\"justify\"\u003eThis kit includes a rapid tissue lysis reagent and a PCR amplification master mix, enabling direct lysis of mouse tissue samples (e.g., tail, ear, toe, liver, kidney, etc.) to quickly release genomic DNA. It is also compatible with blood and cell suspension samples. The supernatant from the lysate can be directly added to the PCR reaction system without the need for DNA purification, offering a convenient workflow.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eMoreover, the kit requires only minimal sample input: 2–5 mm² of ear or liver tissue, 1–5 mm of tail, or 1–2 toes are sufficient for successful amplification.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThe included 2× HieffTM Tissue Direct PCR Mix (With Dye) is a ready-to-use 2× concentrated PCR master mix containing all components required for PCR amplification except template DNA and primers. This greatly simplifies the procedure and reduces the risk of contamination. The mix supports robust amplification of DNA fragments up to 10 kb in length.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThis kit is ideal for applications such as transgenic screening and mouse genotyping.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eLong Amplicon Capability:\u003c\/strong\u003e Supports direct amplification of DNA fragments up to 10 kb.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRapid Extension:\u003c\/strong\u003e Fast extension rate of 5–10 seconds per kb, significantly shortening PCR run time.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Inhibitor Tolerance:\u003c\/strong\u003e Compatible with up to 20% cell lysate in the final reaction mix.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFlexible Lysis Options: \u003c\/strong\u003eEfficient sample lysis in just 3 min at 95°C or 15 min at 60°C.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMultiplex Ready: \u003c\/strong\u003eEnables robust 5–7 plex amplification in a single reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStable \u0026amp; Reliable:\u003c\/strong\u003e Both the kit reagents and lysates demonstrate excellent stability for consistent performance.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellspacing=\"9\" style=\"width: 100.045%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"327\" valign=\"top\" style=\"width: 25.4993%;\"\u003e\n\u003cp\u003eComponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"113\" valign=\"top\" style=\"width: 17.7659%;\"\u003e\n\u003cp\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"120\" valign=\"top\" style=\"width: 17.1389%;\"\u003e\n\u003cp\u003e10191ES20\u003c\/p\u003e\n\u003cp\u003e(20 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"120\" valign=\"top\" style=\"width: 17.5569%;\"\u003e\n\u003cp\u003e10191ES50\u003c\/p\u003e\n\u003cp\u003e(50 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"115\" valign=\"top\" style=\"width: 14.8398%;\"\u003e\n\u003cp\u003e10191ES70\u003c\/p\u003e\n\u003cp\u003e(200 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"327\" valign=\"top\" style=\"width: 25.4993%;\"\u003e\n\u003cp\u003e10191-A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"113\" valign=\"center\" style=\"width: 17.7659%;\"\u003e\n\u003cp\u003eLysis Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"120\" valign=\"center\" style=\"width: 17.1389%;\"\u003e\n\u003cp\u003e1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"120\" valign=\"center\" style=\"width: 17.5569%;\"\u003e\n\u003cp\u003e2 x 1.25 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"115\" valign=\"center\" style=\"width: 14.8398%;\"\u003e\n\u003cp\u003e10 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"327\" valign=\"top\" style=\"width: 25.4993%;\"\u003e\n\u003cp\u003e10191-B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"113\" valign=\"center\" style=\"width: 17.7659%;\"\u003e\n\u003cp\u003eTermination Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"120\" valign=\"center\" style=\"width: 17.1389%;\"\u003e\n\u003cp\u003e1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"120\" valign=\"center\" style=\"width: 17.5569%;\"\u003e\n\u003cp\u003e2 x 1.25 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"115\" valign=\"center\" style=\"width: 14.8398%;\"\u003e\n\u003cp\u003e10 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"327\" valign=\"top\" style=\"width: 25.4993%;\"\u003e\n\u003cp\u003e10191-C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"113\" valign=\"center\" style=\"width: 17.7659%;\"\u003e\n\u003cp\u003e2×Hieff™ Tissue Direct PCR Mix(With Dye)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"120\" valign=\"center\" style=\"width: 17.1389%;\"\u003e\n\u003cp\u003e500 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"120\" valign=\"center\" style=\"width: 17.5569%;\"\u003e\n\u003cp\u003e1.25 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"115\" valign=\"top\" style=\"width: 14.8398%;\"\u003e\n\u003cp\u003e5 x 1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003eUnopened kit: Store at –25°C ~ –15°C. Stable for 12 months.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eAfter opening:\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eComponents A and B: Store at 4°C. Stable for 12 months. For frequent long-term use, aliquot to avoid cross-contamination.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eComponent C: Store at –25°C ~ –15°C. Avoid repeated freeze-thaw cycles. Stable for 12 months.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003emouse genotyping; transgenic screening; direct amplification PCR.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. \u003c!--[endif]--\u003eLong Amplicon Capability: Up to 10 kb.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"Figure 1. Supports Direct Mouse PCR up to 10 kb\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_b93731ae-b743-4c14-b2a8-e451ffa3f10f_1024x1024.png?v=1763702239\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 1. Supports Direct Mouse PCR up to 10 kb\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eMouse tail, ear, toe, and kidney tissues were lysed, and 1 μL of lysate was used to amplify 1–10 kb fragments. Yeasen 10191ES employed a rapid lysis protocol (95 °C for 3 min) and fast extension (5–10 sec\/kb), while other brands followed their respective manuals. Results show that even under rapid lysis and fast cycling, Yeasen-10191ES reliably amplifies fragments up to 10 kb.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. \u003c!--[endif]--\u003eRobust Amplification Across 30–70% GC Content\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"Figure 2. Robust Amplification Across 30–70% GC Content\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_f95b1b7a-f698-4a49-ba83-5b003f18828e_1024x1024.png?v=1763702243\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 2. Robust Amplification Across 30–70% GC Content\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eUsing 1 μL of mouse lysate, 1–10 kb fragments with varying GC content were amplified. Results indicate that 10191ES supports efficient direct PCR of fragments up to 10 kb across a GC range of 30%–70%.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. \u003c!--[endif]--\u003eInhibitor tolerance\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"Figure 3. Excellent PCR Tolerance to Crude Lysate\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/6_d72f04cd-b29a-484e-a233-5a17d6854802_1024x1024.png?v=1763702243\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 3. Excellent PCR Tolerance to Crude Lysate\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eDifferent volumes of lysate prepared at 95 °C for 3 min were added to 20 μL PCR reactions, followed by amplification of 1–10 kb fragments at 5–10 sec\/kb extension. Data show strong tolerance of PCR components to crude lysate, with lysate input up to 20% of the reaction volume.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e4. \u003c!--[endif]--\u003eCompatible with Multiplex PCR\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/7_09c9349f-47c8-4ed3-bba9-6bafa82c7659_1024x1024.png?v=1763702243\" alt=\"Figure 4. Multiplex amplification performance\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 4. Multiplex amplification performance\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eLysates prepared at 95 °C for 3 min were used for 7-plex amplification in 25 μL PCR reactions with a 5 sec\/kb extension rate. Results demonstrate that 10191ES enables multiplex amplification of seven targets ranging from 500 bp to 5 kb.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e5. \u003c!--[endif]--\u003eStable Lysate for Long-Term Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/8_8d71c5ce-aafb-49af-a86e-4ac35c247545_1024x1024.png?v=1763702243\" alt=\"Figure 5. Stability testing of lysate.\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 5. Stability testing of lysate.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eMouse tail lysates were stored at 25 °C or 4 °C for four weeks and used to amplify 1–10 kb fragments. Compared with freshly prepared lysates, stored lysates still supported successful amplification, indicating good stability.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e6. \u003c!--[endif]--\u003eStrong stability\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/9_9b542475-ada9-47eb-879a-f64de3342f09_1024x1024.png?v=1763702244\" alt=\"Figure 6. Excellent Kit Stability\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003eFigure 6. Excellent Kit Stability\u003c\/p\u003e\n\u003cp align=\"justify\"\u003eThe complete 10191ES kit was stored for four weeks at 25 °C or 4 °C, and for two weeks at 37 °C. Amplification of 1–10 kb fragments confirmed that the kit maintains high stability and reliable performance after storage.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10191-MSDS-HB251121.pdf?v=1763702526\"\u003e10191_MSDS_HB251121_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10191ES-Manual-Ver.EN20251118.pdf?v=1763702526\"\u003e10191_Manual_HB20251118.pdf\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"20 T","offer_id":53331732005229,"sku":"10191ES20","price":55.0,"currency_code":"USD","in_stock":true},{"title":"50 T","offer_id":53332191215981,"sku":"10191ES50","price":85.0,"currency_code":"USD","in_stock":true},{"title":"200 T","offer_id":53332191248749,"sku":"10191ES70","price":255.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/4_1024x1024_67d3bf60-3d73-452c-949c-45310195eba3.png?v=1778795252","url":"https:\/\/www.ebiohippo.com\/products\/mouse-tissue-direct-pcr-kit-pro-long-pcr-bht20800005","provider":"BioHippo","version":"1.0","type":"link"}