{"product_id":"mouse-tissue-direct-pcr-kit-with-dye-bht20800002","title":"Mouse Tissue Direct PCR Kit (With Dye)","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis kit can directly and quickly conduct PCR amplification of mouse tissue (such as mouse tail, mouse ear, mouse toe, muscle, etc.), and has strong sample compatibility. Equipped with a powerful lysis buffer, this kit can rapidly lyse samples and release genomic DNA. The lysate can be directly added to the PCR reaction system without purification, and the operation is convenient. In addition, this kit requires low sample input, and 5 mg mouse tissue or 1-5 mm mouse tail can be used for experiments.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThe 2× Mouse Direct PCR Mix provided by this kit is a hot-start PCR reaction solution with a 2-fold concentration. It contains all the components used for PCR amplification except the template and primers, which greatly simplifies the operation process and reduces the chance of contamination. The kit can be used for transgene identification, mouse genotyping, etc.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003eFeatures\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\" align=\"justify\"\u003e\n\u003cspan\u003eLess sample required: 5 mg of mouse tissue or 1-5 mm of mouse tail\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" align=\"justify\"\u003eConvenient template preparation: no need to grind, no need to refine and purify DNA, higher flux, time and money saving\u003cspan style=\"font-size: 0.875rem;\"\u003e.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" align=\"justify\"\u003eOptimized PCR system: with higher specificity and stronger tolerance of PCR reaction inhibitor\u003cspan style=\"font-size: 0.875rem;\"\u003e.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003eApplications\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\" align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eIdentification of transgenic mice\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" align=\"justify\"\u003eGenotyping of mice\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" align=\"justify\"\u003eMouse gene knockout analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003eComponents\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable style=\"width: 99.9572%;\" class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20.9942%;\" width=\"109\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNo.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 35.3681%;\" width=\"365\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.7214%;\" width=\"168\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e10185ES50\u003c\/span\u003e\u003cspan\u003e (50 T)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.915%;\" width=\"175\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e10185ES70\u003c\/span\u003e\u003cspan\u003e (200 T)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20.9942%;\" width=\"109\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10185\u003c\/span\u003e\u003cspan\u003e-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 35.3681%;\" width=\"365\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eBuffer ML\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.7214%;\" width=\"168\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e5×1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.915%;\" width=\"175\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e20×1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20.9942%;\" width=\"109\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003ca name=\"OLE_LINK6\"\u003e\u003c\/a\u003e10185\u003c\/span\u003e\u003cspan\u003e-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 35.3681%;\" width=\"365\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eBuffer MT\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.7214%;\" width=\"168\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e0.6 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.915%;\" width=\"175\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2×1.25 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 20.9942%;\" width=\"109\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10185\u003c\/span\u003e\u003cspan\u003e-C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 35.3681%;\" width=\"365\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2× Mouse Direct PCR Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.7214%;\" width=\"168\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.915%;\" width=\"175\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e2×1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003ea) Buffer ML is a lysis buffer containing strong protein denaturants, please wear gloves.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eb) Buffer MT is a stop buffer used to stop the lysis function of Buffer ML.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003ec) 2× Mouse Direct PCR Mix: Contains hot-start Taq DNA polymerase, dNTP mix, MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, reaction buffer, PCR reaction enhancer, optimizer, stabilizer, electrophoresis indicator dye, etc.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003eSpecifications\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"259\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eProduct specification\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"362\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eKit\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"259\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHot Start\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"362\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eBuilt-in Hot Start\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"259\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eOverhang\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"362\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eBlunt\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"259\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eConditions for transportation\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"362\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eIce Packs\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"259\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eProduct Type\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"362\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDirect PCR Kit\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"259\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eApply to (application)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"362\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eMouse tail, Mouse ear, Rat toe, Viscera, Skin, etc.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003eStorage\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1. Component A: The product should be stored at 2-8℃ for one year. For multiple use for a long time, please avoid cross-contamination.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2. Component B\/C: The product should be stored at -25 ~ -15℃ for one year. Please avoid repeated freeze-thaw.\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003eFigures\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1. Target gene (within 1 kb) amplification result\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 1. Suitable for target gene amplification within 1 kb.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10185-1_1024x1024.png?v=1744348550\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eFigure 1. Suitable for target gene amplification within 1 kb.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2. The extension speed demostration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 2. 500 bp gene, the extension speed can be as fast as 1 sec\/kb.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10185-2_1024x1024.png?v=1744348550\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eFigure 2. 500\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ebp gene, the extension speed can be as fast as 1\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esec\/kb.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10185-3.png?v=1744348550\"\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCited from \"TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell . 2022 Mar 17;185(6):980-994.e15. doi: 10.1016\/j.cell.2022.02.010. \"\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"top_tabc\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" data-v-444b0c7e=\"\"\u003eFAQ\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: Are the sample lysis temperatures for 10185 (Mouse Tissue Direct PCR Kit with Dye) and 10181 (Mouse Tissue Direct PCR Kit) the same?\u003c\/p\u003e\n\u003cp\u003eA: They are all different. The differences in sample lysis temperatures are as follows: 10185: 95℃, 10-30 minutes. 10181: 65℃, 10-30 minutes.\u003c\/p\u003e\n\u003cp\u003eDissociation operation differences: 10185: Dissociate component A and termination dissociation component B separately, and mix them after dissociation is complete. 10181: Add dissociation component A and termination dissociation component B together, but the termination dissociation requires 5 minutes.\u003c\/p\u003e\n\u003cp\u003eQ: For direct PCR on mouse tissues, is it better to use mouse toes or mouse tails? Is purification necessary?\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eA: No need for purification. Centrifuge to obtain the supernatant (optional). There is no comparison between the effects of mouse tail and mouse toe. Usually, mouse tail is used.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"top_tabc\"\u003e\n\u003cstrong\u003e\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" data-v-444b0c7e=\"\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\n\u003c\/h3\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eSafety Data Sheet\u003cstrong\u003e\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10185_MSDS_HB220420_EN_1652931562600.PDF\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003cimg alt=\"\" data-v-98c835fc=\"\"\u003e\u003c\/a\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10185-MSDS-HB250721.pdf?v=1774491086\" target=\"_blank\" title=\"download\" rel=\"noopener\"\u003e10185_MSDS_HB250721\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10185-MSDS-HB250721.pdf?v=1753170436\" target=\"_blank\"\u003e.PDF\u003c\/a\u003e\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10185_MSDS_HB220420_EN_1652931562600.PDF\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003c\/a\u003e\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/10185_MSDS_HB220420_EN_1652931562601.PDF\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManual\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/10185_Manual_HB230603_EN.pdf\" data-v-98c835fc=\"\" target=\"_blank\"\u003e\u003cimg alt=\"\" data-v-98c835fc=\"\"\u003e\u003c\/a\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10185-Manual-Ver.EN20250513.pdf?v=1753170433\" target=\"_blank\"\u003e10185_Manual_Ver.EN20250513.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"list\"\u003e\n\u003cstrong\u003e\u003cspan data-v-444b0c7e=\"\" id=\"tabCBox3\" class=\"boxTap\"\u003eCitations \u0026amp; References:\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003e[1] Luo J, Yang Q, Zhang X, et al. TFPI is a colonic crypt receptor for TcdB from hypervirulent clade 2 C. difficile. Cell. 2022;185(6):980-994.e15. doi:10.1016\/j.cell.2022.02.010(IF:41.584)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e[2] Zhao J, Chen J, Li YY, Xia LL, Wu YG. Bruton's tyrosine kinase regulates macrophage‑induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021;48(3):177. doi:10.3892\/ijmm.2021.5010(IF:4.101)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan class=\"boxTap\" id=\"tabCBox2\" data-v-444b0c7e=\"\"\u003eFAQs:\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eCommon problem\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003ePossible reason\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eSolution\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\"\u003e\n\u003cp\u003e\u003cspan\u003eThere were no bands in the positive control and the samples to be tested.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eThe PCR reaction system or reaction conditions were not suitable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eUse gradient PCR to explore the optimal reaction conditions for PCR.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eImproper storage of PCR reagents loses activity.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003e2× Mouse Direct PCR Mix should be stored at -20°C and avoid repeated freezing and thawing during use. If used frequently, it can be stored at 4°C for a short time.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePrimer design issues.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eTry redesigning the primers to check.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"5\"\u003e\n\u003cp\u003e\u003cspan\u003eThe positive control has a band of interest, and the sample to be tested has no band or a weak band.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eImproper storage or prolonged storage can cause loss of reagent activity.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eUse fresh reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eAdd tissue lysate in excess.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eIncrease the reaction system, or reduce the amount of lysate.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eThe sample lysis mixture has been stored improperly or stored for too long, and the DNA genome has been degraded.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eThe lysate mixture can be stored at 4°C for 2-3 days. Try to use a freshly prepared lysate mixture for PCR.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eThe amount of template added is not suitable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eThe amount of template added was optimized within the range of 1-10% of the reaction system.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eInsufficient number of PCR cycles.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eIncrease the number of PCR cycles, preferably 35-40 cycles. Because of the complexity of the template, PCR reactions should be performed with 5-10 more cycles than with purified DNA template.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"3\"\u003e\n\u003cp\u003e\u003cspan\u003eNonspecific amplification\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePCR annealing temperature too low, cycle number, primer concentration, or template concentration too high.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eIncrease PCR annealing temperature and decrease PCR cycle number, primer concentration or template concentration.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003ePCR primer mismatches.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eRedesign PCR primers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eThe temperature is too high when preparing the PCR reaction system or the time is too long after the preparation is completed.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eThe preparation of the PCR reaction system is carried out at low temperature, and the PCR amplification reaction is carried out as soon as possible after the preparation is completed.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"2\"\u003e\n\u003cp\u003e\u003cspan\u003eTarget band appears in negative control\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eContamination of operating tools or reagents.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eAll reagents or equipment in the experiment should be autoclaved. Be careful and gentle when handling to prevent the target sequence from being sucked into the sample gun or spilled out of the centrifuge tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eCross-contamination between samples.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp\u003e\u003cspan\u003eEach sampler is only used for one sample; or after taking one sample, immerse the edge of the sampler in 2% sodium hypochlorite solution, rinse repeatedly, and then dry the residue with a clean paper towel.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan class=\"boxTap\" id=\"tabCBox3\" data-v-444b0c7e=\"\"\u003e\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"50 T","offer_id":53332189544813,"sku":"10185ES50","price":65.0,"currency_code":"USD","in_stock":true},{"title":"200 T","offer_id":53332189806957,"sku":"10185ES70","price":295.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10185-1_1024x1024_a9f5efab-41cb-4f31-84da-4b74381db9a1.png?v=1778795250","url":"https:\/\/www.ebiohippo.com\/products\/mouse-tissue-direct-pcr-kit-with-dye-bht20800002","provider":"BioHippo","version":"1.0","type":"link"}