{"product_id":"mpeg1-antibody-macrophage-expressed-gene-1-perforin-2-bha17135368","title":"MPEG1 Antibody \/ Macrophage-expressed gene 1 \/ Perforin 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMPEG1 Antibody \/ Macrophage-expressed gene 1 \/ Perforin 2 is a anti-MPEG1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MPEG1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMPEG1 antibody detects Macrophage-expressed gene 1 protein, also known as Perforin-2, a pore-forming immune effector that contributes to host defense against intracellular pathogens. MPEG1 belongs to the MACPF (membrane-attack-complex\/perforin) superfamily of pore-forming proteins, which includes complement components and cytotoxic lymphocyte perforins. The MPEG1 antibody is an essential research tool for investigating innate immunity, host-pathogen interactions, and macrophage antimicrobial mechanisms that protect cells from bacterial infection.\u003cbr\u003e\u003cbr\u003eMPEG1 is encoded by the MPEG1 gene on human chromosome 11q12.1. The protein contains a signal peptide, an N-terminal MACPF domain responsible for membrane pore formation, and a C-terminal transmembrane region that anchors it to endosomal and plasma membranes. MPEG1 is predominantly expressed in macrophages, dendritic cells, and other phagocytes, where it localizes to endolysosomal compartments. Upon bacterial invasion, MPEG1 is upregulated and translocated to pathogen-containing vacuoles, where it inserts into microbial membranes to mediate lysis.\u003cbr\u003e\u003cbr\u003eThe MPEG1 antibody reveals an ~79 kDa band in western blot corresponding to full-length MPEG1\/Perforin-2. Immunofluorescence microscopy demonstrates punctate cytoplasmic and vesicular localization consistent with its endosomal distribution. Functional studies have shown that loss of MPEG1 impairs bacterial killing and increases susceptibility to infection. It acts downstream of pattern-recognition receptor activation, integrating with pathways controlled by NF-?B and interferon signaling. MPEG1 also interacts with ESCRT-associated and autophagic machinery to remove damaged or infected vacuoles.\u003cbr\u003e\u003cbr\u003eIn addition to antimicrobial defense, MPEG1 contributes to tissue homeostasis and inflammation control. It modulates cytokine production and can influence antigen presentation by professional phagocytes. Structural analyses reveal that MPEG1 forms ring-like oligomers that perforate target membranes, similar to complement MAC pores. The protein's activity is tightly regulated to prevent host cell damage.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MPEG1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MPEG1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MPEG1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MPEG1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MPEG1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290298733,"sku":"FY12465","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c19bb278-3658-499a-8a31-61f78f017c7d.jpg?v=1782237013","url":"https:\/\/www.ebiohippo.com\/products\/mpeg1-antibody-macrophage-expressed-gene-1-perforin-2-bha17135368","provider":"BioHippo","version":"1.0","type":"link"}