{"product_id":"mplkip-antibody-m-phase-specific-plk1-interacting-protein-bha17135233","title":"MPLKIP Antibody \/ M phase specific PLK1 interacting protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMPLKIP Antibody \/ M phase specific PLK1 interacting protein is a anti-MPLKIP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MPLKIP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMPLKIP antibody is used to study M phase specific PLK1 interacting protein, a small nuclear protein that binds the mitotic kinase PLK1 and helps coordinate chromosome dynamics and cell cycle progression. By engaging Polo like kinase 1 during M phase, the protein contributes to proper spindle function and timely transition through mitosis, thereby preserving genome integrity. Beyond cell division, M phase specific PLK1 interacting protein participates in gene regulatory programs and has been linked to transcription-coupled processes that safeguard cell identity and stress adaptation.\u003cbr\u003e\u003cbr\u003eThe protein localizes to the nucleus and perinuclear regions, where it interfaces with chromatin and cell cycle machinery. Loss of function mutations in the MPLKIP gene, historically known as TTDN1, cause trichothiodystrophy non photosensitive type 1, a disorder characterized by brittle hair and developmental features, underscoring its role in nuclear homeostasis. Experimental systems suggest that M phase specific PLK1 interacting protein acts as a scaffold that positions kinases and effectors to ensure accurate phosphorylation timing, with downstream consequences for cohesion, condensation, and checkpoint signaling.\u003cbr\u003e\u003cbr\u003eIn the laboratory, researchers use MPLKIP antibody for immunoblotting to monitor expression across the cell cycle, for immunofluorescence to visualize nuclear patterns in interphase and mitosis, and for co-immunoprecipitation to examine complexes with PLK1 and associated regulators. When paired with synchronization, kinase inhibitors, or auxin-degron depletion, the antibody supports dissection of cause-and-effect relationships between protein abundance, phosphorylation events, and mitotic fidelity. In disease models, MPLKIP antibody enables evaluation of variant impact on stability and localization, clarifying genotype-phenotype mechanisms relevant to neurodevelopment and ectodermal biology.\u003cbr\u003e\u003cbr\u003eThe MPLKIP antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MPLKIP - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MPLKIP - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MPLKIP - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MPLKIP - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MPLKIP+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047284695405,"sku":"FY12330","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2f4b13d5-5d49-4d9e-84aa-7f2f47c10176.jpg?v=1782236992","url":"https:\/\/www.ebiohippo.com\/products\/mplkip-antibody-m-phase-specific-plk1-interacting-protein-bha17135233","provider":"BioHippo","version":"1.0","type":"link"}