{"product_id":"mre-reporter-lentivirus-bhv19400038","title":"MRE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe metal response element (MRE) is the DNA sequence recognized by metal-regulatory transcription factor 1 (MTF-1), a zinc-finger transcription factor that serves as the central sensor of cellular metal status. When intracellular concentrations of zinc and other heavy metals rise, MTF-1 binds MRE sequences in target gene promoters and activates transcription. Its principal targets include the metallothionein genes, which encode small cysteine-rich proteins that chelate metal ions and protect cells from metal toxicity and oxidative stress. The MTF-1\/MRE pathway is essential for metal homeostasis and the cellular response to heavy metal exposure, and it is studied in the contexts of metal toxicology, oxidative stress, and cell signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe MRE Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of MTF-1 and metal response pathway activity in human and mouse cells. The construct contains tandem repeats of the metal response element placed upstream of a minimal promoter driving the reporter gene (GFP, RFP, firefly luciferase, or Renilla luciferase), with an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to studying metal-induced transcription and MTF-1 activity in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Metal response pathway (MTF-1) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251620602221,"sku":"LTV-0042-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310895030637,"sku":"LTV-0042-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310895063405,"sku":"LTV-0042-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310895096173,"sku":"LTV-0042-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310895128941,"sku":"LTV-0042-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310895161709,"sku":"LTV-0042-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310895194477,"sku":"LTV-0042-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_f40ab3f1-3646-446d-9b43-8bb0d116e75c.jpg?v=1776934332","url":"https:\/\/www.ebiohippo.com\/products\/mre-reporter-lentivirus-bhv19400038","provider":"BioHippo","version":"1.0","type":"link"}