{"product_id":"msh2-over-expressing-nalm-6-cell-line-nalm-6-msh2-positive-bhc10923247","title":"MSH2 Over-expressing Nalm-6 Cell Line (Nalm-6 MSH2+)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe MSH2 Over-expressing Nalm-6 Cell Line (Nalm-6 MSH2+) is a genetically modified version of the Nalm-6 human leukemia cell line, engineered to over-express the super-exon of MSH2 gene. MSH2 is a key component of the DNA mismatch repair (MMR) system, which is essential for maintaining genomic stability by correcting DNA replication errors.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e MSH2 Over-expressing Nalm-6 Cell Line (Nalm-6 MSH2+) is supplied as a tumor cell line derived from Human peripheral blood.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Suspension, lymphocyte-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS (Regular*) + 1X Non-Essential Amino Acids (TM068) + 1mM Sodium Pyruvate (TM057) + 0.15 µM Vitamin B12 + 50 µM 2-Mercaptoethanol (CH045) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 1.0 mg\/ml Geneticin\/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe overexpression of MSH2 in this cell line provides a model to study the effects of enhanced DNA repair on genomic stability, cell cycle regulation, and response to DNA-damaging agents. This stable cell line is useful for investigating the role of MSH2 in leukemogenesis, DNA repair mechanisms, and the development of therapeutic strategies targeting the MMR pathway. Donor\/background information provided for this product: Male, 19, Lymphoblastic leukemia.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS (Regular*) + 1X Non-Essential Amino Acids (TM068) + 1mM Sodium Pyruvate (TM057) + 0.15 µM Vitamin B12 + 50 µM 2-Mercaptoethanol (CH045) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 1.0 mg\/ml Geneticin\/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:6-1:10\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180553101677,"sku":"T6065","price":710.7,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/4x0xJViyIPoiKS0ZPkmSWq7PJf7HyI5JOGDqy5bO.png?v=1774957884","url":"https:\/\/www.ebiohippo.com\/products\/msh2-over-expressing-nalm-6-cell-line-nalm-6-msh2-positive-bhc10923247","provider":"BioHippo","version":"1.0","type":"link"}