{"product_id":"msi2-antibody-musashi-rna-binding-protein-2-bha17135461","title":"MSI2 Antibody \/ Musashi RNA-binding protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMSI2 Antibody \/ Musashi RNA-binding protein 2 is a anti-MSI2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MSI2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMSI2 antibody detects Musashi RNA-binding protein 2, an evolutionarily conserved RNA-binding protein that regulates mRNA translation and stem cell maintenance. MSI2 belongs to the Musashi family, which controls post-transcriptional gene expression during development and cancer progression. The MSI2 antibody is widely used in stem cell biology, leukemia research, and studies of neural differentiation to examine mRNA regulation and self-renewal mechanisms.\u003cbr\u003e\u003cbr\u003eMSI2 is encoded by the MSI2 gene located on human chromosome 17q23.2. The protein is approximately 38 kilodaltons and contains two RNA recognition motifs (RRMs) at the N-terminus that specifically bind to target mRNAs containing UAG motifs in their 3' untranslated regions. Through this interaction, MSI2 represses translation of transcripts involved in differentiation while enhancing those promoting proliferation and stemness. MSI2 expression is particularly high in hematopoietic stem cells and neural progenitors.\u003cbr\u003e\u003cbr\u003eThe MSI2 antibody detects a 38 kilodalton band by western blot and shows cytoplasmic and perinuclear staining in stem cell populations and tumor cells. MSI2 regulates asymmetric cell division and maintains an undifferentiated state by antagonizing translational repressors such as PABP and interacting with the translational initiation machinery. In hematopoiesis, MSI2 cooperates with HOXA9 and MYC signaling to promote leukemic transformation. Overexpression correlates with poor prognosis in acute myeloid leukemia, breast cancer, and colorectal carcinoma.\u003cbr\u003e\u003cbr\u003eIn the nervous system, MSI2 modulates neurogenesis by controlling translation of key developmental regulators such as NUMB and REST. Loss of MSI2 leads to premature differentiation and reduced progenitor proliferation. Its function is tightly regulated by phosphorylation and interaction with signaling pathways including Notch and mTOR.\u003cbr\u003e\u003cbr\u003eBecause of its dual role in maintaining stemness and driving oncogenesis, MSI2 serves as a key marker and therapeutic target in cancer stem cell biology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MSI2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MSI2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MSI2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MSI2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MSI2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291937133,"sku":"FY12558","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9875256c-6996-48e6-85ad-443bb5334034.jpg?v=1782237017","url":"https:\/\/www.ebiohippo.com\/products\/msi2-antibody-musashi-rna-binding-protein-2-bha17135461","provider":"BioHippo","version":"1.0","type":"link"}