{"product_id":"msl1-antibody-male-specific-lethal-1-bha17135477","title":"MSL1 Antibody \/ Male-specific lethal 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMSL1 Antibody \/ Male-specific lethal 1 is a anti-MSL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MSL1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMSL1 antibody detects Male-specific lethal 1 homolog, a chromatin-associated protein that forms part of the MSL (male-specific lethal) complex responsible for histone acetylation and dosage compensation. Originally discovered in Drosophila, the human MSL complex includes MSL1, MSL2, MSL3, and the histone acetyltransferase KAT8 (also known as MOF). Together, these proteins catalyze histone H4 lysine 16 acetylation, a mark associated with transcriptional activation. The MSL1 antibody is widely used in chromatin biology and epigenetic regulation studies to explore histone modification and gene dosage balance.\u003cbr\u003e\u003cbr\u003eMSL1 is encoded by the MSL1 gene located on human chromosome Xq28. The protein is approximately 618 amino acids long and contains coiled-coil regions that mediate interactions with MSL2 and MSL3, stabilizing the MSL complex structure. MSL1 localizes primarily to euchromatic regions of the nucleus and contributes to the spreading of acetylated histone marks along active genes. The protein serves as a scaffold, ensuring the recruitment and correct assembly of other MSL subunits onto chromatin.\u003cbr\u003e\u003cbr\u003eThe MSL1 antibody detects a 75 kilodalton band by western blot and demonstrates nuclear staining in cells undergoing active transcription. Through its role in facilitating KAT8-dependent histone H4 acetylation, MSL1 influences global chromatin accessibility and transcriptional output. Loss of MSL1 disrupts histone acetylation patterns and leads to altered gene expression profiles, particularly affecting dosage-sensitive genes on the X chromosome.\u003cbr\u003e\u003cbr\u003eBeyond its role in dosage compensation, MSL1 participates in general transcriptional regulation across autosomes. It has been linked to stem cell differentiation, stress responses, and genome organization. Abnormal expression or mutation of MSL1 alters chromatin structure and is associated with cancer and neurodevelopmental disorders. As a regulator of chromatin state, MSL1 bridges histone modification machinery with transcriptional control mechanisms that shape epigenetic identity.\u003cbr\u003e\u003cbr\u003eBecause of its central function in chromatin remodeling and transcriptional activation, MSL1 serves as a valuable marker for studies of epigenetic regulation, gene dosage balance, and histone acetylation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MSL1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MSL1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MSL1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MSL1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MSL1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292854637,"sku":"FY12574","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_fb2cbe91-1fc9-41f2-b531-40cf8473d4c6.jpg?v=1782237062","url":"https:\/\/www.ebiohippo.com\/products\/msl1-antibody-male-specific-lethal-1-bha17135477","provider":"BioHippo","version":"1.0","type":"link"}