{"product_id":"mst1-antibody-mammalian-ste20-like-protein-kinase-1-stk4-bha17135877","title":"MST1 Antibody \/ Mammalian STE20-like protein kinase 1 \/ STK4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMST1 Antibody \/ Mammalian STE20-like protein kinase 1 \/ STK4 is a anti-MST1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MST1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMST1 antibody detects Mammalian STE20-like protein kinase 1, a serine\/threonine kinase that functions as a central regulator of apoptosis, stress signaling, and organ size control. The UniProt recommended name is Serine\/threonine-protein kinase 4 (STK4), commonly referred to as MST1. This kinase belongs to the Hippo signaling pathway, a conserved network that restricts cell proliferation and promotes programmed cell death to maintain tissue homeostasis.\u003cbr\u003e\u003cbr\u003eFunctionally, MST1 antibody identifies a 487-amino-acid cytoplasmic kinase that becomes activated in response to oxidative stress, DNA damage, or cell detachment. Upon activation, MST1 autophosphorylates and phosphorylates downstream kinases such as LATS1\/2, initiating a signaling cascade that culminates in the phosphorylation and inactivation of the transcriptional co-activators YAP and TAZ. This process suppresses genes promoting cell proliferation and survival. Additionally, MST1 can translocate to the nucleus, where it phosphorylates histone H2B and FOXO transcription factors to promote apoptosis and DNA repair.\u003cbr\u003e\u003cbr\u003eThe MST1 gene is located on chromosome 20q13.12 and encodes a key component of the mammalian Hippo pathway. MST1 activity is tightly regulated through interactions with adaptor proteins including SAV1 and MOB1, which enhance its kinase activity and substrate recognition. The pathway acts as a molecular brake on organ growth and tumorigenesis by balancing proliferation and cell death. Loss or mutation of MST1 disrupts Hippo signaling, leading to uncontrolled tissue growth and increased cancer risk.\u003cbr\u003e\u003cbr\u003eIn addition to its role in growth control, MST1 regulates immune function by modulating T-cell migration and survival. It promotes lymphocyte trafficking by controlling actin cytoskeleton organization and integrin-mediated adhesion. MST1 deficiency in humans results in primary immunodeficiency characterized by recurrent infections and defective T-cell activation. In neurons, MST1 contributes to axonal degeneration under oxidative stress, linking it to neurodegenerative disease mechanisms.\u003cbr\u003e\u003cbr\u003eMST1 antibody is widely used in research on apoptosis, signal transduction, and cancer biology. It is suitable for immunoblotting, immunoprecipitation, and fluorescence microscopy to monitor MST1 activation, phosphorylation, and subcellular localization. In cancer research, MST1 serves as a tumor suppressor marker, while in neuroscience, it is investigated for its role in neuronal apoptosis. The antibody supports studies on kinase cascades that control organ size, cytoskeletal remodeling, and oxidative stress response.\u003cbr\u003e\u003cbr\u003eStructurally, MST1 contains an N-terminal kinase domain and a C-terminal SARAH domain responsible for dimerization and interaction with Hippo pathway components. Post-translational modifications including autophosphorylation, caspase-mediated cleavage, and ubiquitination regulate its activity and localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MST1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MST1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MST1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MST1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MST1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308026221,"sku":"FY12975","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_faa6541d-4adc-47ed-a9f3-aefbb6978922.jpg?v=1782237073","url":"https:\/\/www.ebiohippo.com\/products\/mst1-antibody-mammalian-ste20-like-protein-kinase-1-stk4-bha17135877","provider":"BioHippo","version":"1.0","type":"link"}