{"product_id":"mtch2-antibody-mitochondrial-carrier-homolog-2-bha17135937","title":"MTCH2 Antibody \/ Mitochondrial carrier homolog 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMTCH2 Antibody \/ Mitochondrial carrier homolog 2 is a anti-MTCH2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MTCH2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMTCH2 antibody detects Mitochondrial carrier homolog 2, a mitochondrial outer membrane protein involved in metabolism, apoptosis regulation, and mitochondrial dynamics. The UniProt recommended name is Mitochondrial carrier homolog 2 (MTCH2). Although originally classified as a putative solute carrier, MTCH2 primarily functions as a regulator of mitochondrial morphology and cell death signaling.\u003cbr\u003e\u003cbr\u003eFunctionally, MTCH2 antibody identifies a 305-amino-acid protein localized to the mitochondrial outer membrane. MTCH2 interacts with pro-apoptotic proteins such as BID and regulates mitochondrial outer membrane permeabilization (MOMP) during apoptosis. It modulates the recruitment and activation of truncated BID (tBID), a key step in BAX and BAK activation leading to cytochrome c release and caspase activation. Thus, MTCH2 serves as a checkpoint controlling apoptosis sensitivity.\u003cbr\u003e\u003cbr\u003eThe MTCH2 gene is located on chromosome 11p11.2 and encodes a protein expressed in metabolically active tissues including brain, liver, and skeletal muscle. MTCH2 contains six predicted transmembrane helices and shares limited homology with mitochondrial carrier family proteins, though it lacks classical solute transport function. Instead, it influences mitochondrial bioenergetics and lipid metabolism through protein-protein interactions at the outer membrane.\u003cbr\u003e\u003cbr\u003eIn addition to apoptosis, MTCH2 plays roles in energy metabolism and mitochondrial fission-fusion balance. It has been shown to interact with mitochondrial fission regulator DRP1 and influence cristae remodeling under metabolic stress. Genetic studies have associated MTCH2 variants with obesity and metabolic syndrome, suggesting that it contributes to energy balance by regulating mitochondrial efficiency and lipid oxidation.\u003cbr\u003e\u003cbr\u003eIn neuronal and immune systems, MTCH2 contributes to mitochondrial trafficking and immune cell activation. Overexpression of MTCH2 sensitizes cells to apoptotic stimuli, while its depletion can promote survival under stress conditions. Its dual role in metabolism and apoptosis underscores its function as a metabolic gatekeeper linking mitochondrial function to cell fate decisions.\u003cbr\u003e\u003cbr\u003eMTCH2 antibody is widely used in mitochondrial biology, apoptosis, and metabolism research. It is suitable for western blotting, immunocytochemistry, and co-immunoprecipitation to study MTCH2 localization and interaction networks. This antibody supports studies of mitochondrial outer membrane regulation, lipid metabolism, and programmed cell death. In disease research, it assists in examining MTCH2's contribution to metabolic disorders and neurodegeneration.\u003cbr\u003e\u003cbr\u003eStructurally, MTCH2 contains conserved hydrophobic transmembrane helices that form a scaffold for protein binding. It lacks the canonical carrier signature motifs found in other mitochondrial carriers, reflecting its specialized signaling function.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MTCH2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MTCH2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MTCH2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MTCH2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MTCH2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310188909,"sku":"FY13035","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4e4c8b7f-de03-480a-bb49-0e0ff5313fbf.jpg?v=1782237077","url":"https:\/\/www.ebiohippo.com\/products\/mtch2-antibody-mitochondrial-carrier-homolog-2-bha17135937","provider":"BioHippo","version":"1.0","type":"link"}