{"product_id":"mtmr3-antibody-myotubularin-related-protein-3-bha17135902","title":"MTMR3 Antibody \/ Myotubularin-related protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMTMR3 Antibody \/ Myotubularin-related protein 3 is a anti-MTMR3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MTMR3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMTMR3 antibody detects Myotubularin-related protein 3, a phosphatidylinositol phosphatase involved in autophagy, endosomal trafficking, and phosphoinositide metabolism. The UniProt recommended name is Myotubularin-related protein 3 (MTMR3). This enzyme belongs to the myotubularin family of dual-specificity phosphatases that dephosphorylate phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2], key regulators of endosomal dynamics and membrane trafficking.\u003cbr\u003e\u003cbr\u003eFunctionally, MTMR3 antibody identifies a 911-amino-acid cytoplasmic protein containing a PH-GRAM lipid-binding domain, a catalytic phosphatase domain, and a coiled-coil region responsible for dimerization. MTMR3 acts as a negative regulator of autophagy by dephosphorylating PI3P, thereby modulating membrane dynamics at autophagosome formation sites. It also regulates endosomal sorting and vesicle trafficking between endosomes and the trans-Golgi network.\u003cbr\u003e\u003cbr\u003eThe MTMR3 gene is located on chromosome 22q12.2 and is expressed in a broad range of tissues, including muscle, brain, and kidney. Its activity ensures proper membrane identity and turnover of signaling lipids within endocytic and autophagic pathways. Dysregulation of MTMR3 expression leads to aberrant phosphoinositide signaling, contributing to impaired autophagy, vesicle accumulation, and neurodegeneration.\u003cbr\u003e\u003cbr\u003eIn cellular signaling, MTMR3 integrates nutrient and stress signals to balance autophagy and cell survival. It interacts with mTORC1 and ULK1 complexes, coordinating lipid metabolism with autophagic flux. Loss or inhibition of MTMR3 enhances autophagy initiation, while overexpression suppresses it. In cancer, MTMR3 acts context-dependently, functioning as either a tumor suppressor or survival factor depending on cell type and metabolic state.\u003cbr\u003e\u003cbr\u003eMTMR3 antibody is widely used in autophagy, membrane trafficking, and phosphoinositide signaling research. It is suitable for western blotting, immunofluorescence, and lipid signaling assays to detect MTMR3 expression and localization to endosomal membranes. In neurobiology, this antibody aids in studying lysosomal and endocytic dysfunction associated with neurodegenerative diseases. In cancer studies, MTMR3 detection contributes to understanding metabolic regulation and cell stress adaptation.\u003cbr\u003e\u003cbr\u003eStructurally, MTMR3 contains a catalytic Cys-X5-Arg motif typical of the protein tyrosine phosphatase superfamily, with substrate specificity directed toward phosphatidylinositol derivatives. The PH-GRAM domain mediates lipid recognition, while the coiled-coil region enables dimer formation with other myotubularin family members.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MTMR3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MTMR3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MTMR3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MTMR3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MTMR3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308648813,"sku":"FY13000","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8657c564-260f-449e-9c70-62492fd70a79.jpg?v=1782237077","url":"https:\/\/www.ebiohippo.com\/products\/mtmr3-antibody-myotubularin-related-protein-3-bha17135902","provider":"BioHippo","version":"1.0","type":"link"}