{"product_id":"mtor-antibody-mammalian-target-of-rapamycin-bha17135654","title":"mTOR Antibody \/ Mammalian target of rapamycin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003emTOR Antibody \/ Mammalian target of rapamycin is a anti-mTOR Rabbit antibody Recombinant Rabbit Monoclonal clone 32M82 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e mTOR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32M82, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003emTOR antibody detects mechanistic target of rapamycin, encoded by the MTOR gene. Common alternate names include FRAP1, RAFT1, and RAPT1. mTOR is a serine threonine kinase that integrates nutrient, energy, and growth factor signals to regulate cell growth, metabolism, and survival. As the catalytic core of the mTORC1 and mTORC2 complexes, it influences protein synthesis, lipid metabolism, and cytoskeletal organization. mTOR is highly conserved and functions as a central regulator of cellular homeostasis.\u003cbr\u003e\u003cbr\u003emTOR antibody is widely applied in cancer research, immunology, and metabolism. In cancer, hyperactivation of mTOR pathways drives uncontrolled proliferation and metabolic reprogramming. In immunology, mTOR regulates T cell differentiation and macrophage function. In metabolic biology, mTOR senses nutrient status and controls autophagy. By detecting mTOR protein, researchers can study how this kinase integrates diverse signals to maintain balance or promote disease.\u003cbr\u003e\u003cbr\u003eValidated applications for mTOR antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting reveals mTOR expression and size, immunohistochemistry maps distribution in tumors and tissues, and immunofluorescence highlights subcellular localization at lysosomes and other compartments. These methods provide strong experimental tools to characterize mTOR biology.\u003cbr\u003e\u003cbr\u003eDysregulation of mTOR signaling contributes to cancer, diabetes, cardiovascular disease, and neurodegeneration. Mutations and upstream activation of PI3K and Akt lead to aberrant mTOR activity. Therapeutically, mTOR inhibitors such as rapamycin and its analogs are used to treat cancer and transplantation related immune suppression. Antibody based detection of mTOR supports both mechanistic studies and translational applications.\u003cbr\u003e\u003cbr\u003emTOR antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=mTOR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=mTOR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=mTOR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/mTOR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=mTOR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047299473773,"sku":"FY12751","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_bc09b7f4-8ac4-410d-98a9-60c048ff6e6e.jpg?v=1772019336","url":"https:\/\/www.ebiohippo.com\/products\/mtor-antibody-mammalian-target-of-rapamycin-bha17135654","provider":"BioHippo","version":"1.0","type":"link"}