{"product_id":"mutant-alpha-synuclein-ala30pro-stable-h4-cell-line-bhc10923040","title":"Mutant Alpha-Synuclein (Ala30Pro) Stable H4 Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis cell line stably expresses the disease related mutant (Ala30Pro) of alpha-synuclein (α-synuclein) under tetracycline regulatable expression (tet-off). Therefore, it is useful for studies investigating the role of α-synuclein in Lewy body formation and the development of related neurodegenerative diseases.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Mutant Alpha-Synuclein (Ala30Pro) Stable H4 Cell Line is supplied as an engineered cell line derived from Human brain.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast-like (flat cells with large cytoplasm)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for cell adhesion to the culture vessels. PriGrow C Medium (TM100) + 10% FBS(Regular*) + 200ug\/ml Hygromycin B (G265) + 200ug\/ml G418 (G262) + 1 ug\/ml tetracycline* + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: cells are better at \u0026gt;80% confluency; cells do not grow well if too sparse. These stable lines were developed to have tet regulatable expression (tet-off); stable expression can be maintained with Hygromycin B (200ug\/ml) and G418 (200ug\/ml). *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Luciferase reporter expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eComplete list of cell lines from this panel:Cat. T3427 - Human Wild-Type Alpha-Synuclein H4 Cell LineCat. T3428 - Full-length Gaussia Luciferase H4 Stable Cell Line (HgLuc) Cat. T3429 - α-Synuclein-Luciferase Bioluminescent Stable H4 Cell Line (H4 S1S2)Cat. T3430 - α-Synuclein-Venus Fluorescent Stable H4 Cell Line (H4 V1S\/SV2)Cat. T3441 - Full-length Venus Expressing H4 Stable Cell Line (HgLuc) Cat. T3458 - Mutant Alpha-Synuclein (Ala53Thr) Stable H4 Cell LineCat. T3459 - Mutant Alpha-Synuclein (Ala30Pro) Stable H4 Cell Line\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for cell adhesion to the culture vessels. PriGrow C Medium (TM100) + 10% FBS(Regular*) + 200ug\/ml Hygromycin B (G265) + 200ug\/ml G418 (G262) + 1 ug\/ml tetracycline* + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: cells are better at \u0026gt;80% confluency; cells do not grow well if too sparse. These stable lines were developed to have tet regulatable expression (tet-off); stable expression can be maintained with Hygromycin B (200ug\/ml) and G418 (200ug\/ml). *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisclaimer:\u003c\/strong\u003e \u003cp\u003e1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.\u003c\/p\u003e\n\u003cp\u003e2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.\u003c\/p\u003e\n\u003cp\u003e3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).\u003c\/p\u003e\n\u003cp\u003e4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic application(s). Please contact a technical service representative for more information.\u003c\/p\u003e\n\u003cp\u003e5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.\u003c\/p\u003e\n\u003cp\u003e6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period.\"\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180549529965,"sku":"T3459","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/uAXspC0ZuRZ0ZZuEKO6q8igBq9YfpWprGnOtghdK.png?v=1774957877","url":"https:\/\/www.ebiohippo.com\/products\/mutant-alpha-synuclein-ala30pro-stable-h4-cell-line-bhc10923040","provider":"BioHippo","version":"1.0","type":"link"}