{"product_id":"mvk-antibody-mevalonate-kinase-bha17135535","title":"MVK Antibody \/ Mevalonate kinase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eMVK Antibody \/ Mevalonate kinase is a anti-MVK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e MVK\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eMVK antibody detects Mevalonate kinase, a key enzyme in the mevalonate pathway responsible for cholesterol and isoprenoid biosynthesis. MVK catalyzes the phosphorylation of mevalonic acid to 5-phosphomevalonate, a crucial step in the production of sterols and nonsterol isoprenoids. The MVK antibody is widely used in metabolic, genetic, and biochemical research to study cholesterol regulation, inflammation, and metabolic disorders.\u003cbr\u003e\u003cbr\u003eMVK is encoded by the MVK gene located on human chromosome 12q24.11. The protein is approximately 396 amino acids long and localizes to the peroxisome and cytoplasm. MVK activity is regulated by feedback inhibition through cholesterol and isoprenoid intermediates, ensuring metabolic balance in lipid biosynthesis. The enzyme participates in the early steps of the mevalonate pathway, linking carbohydrate metabolism to lipid production.\u003cbr\u003e\u003cbr\u003eThe MVK antibody detects a 43 kilodalton protein by western blot and reveals diffuse cytoplasmic and peroxisomal staining under immunofluorescence microscopy. MVK provides precursors for essential biomolecules such as coenzyme Q, dolichol, and prenylated proteins, which are required for membrane integrity, protein glycosylation, and signal transduction.\u003cbr\u003e\u003cbr\u003eMutations in MVK cause mevalonic aciduria and hyperimmunoglobulinemia D syndrome, characterized by recurrent fever and inflammation due to impaired isoprenoid synthesis and excessive accumulation of mevalonic acid. MVK deficiency disrupts protein prenylation, mitochondrial function, and immune regulation, leading to autoinflammatory symptoms. The enzyme's activity is thus essential for cellular metabolism, immune homeostasis, and energy balance.\u003cbr\u003e\u003cbr\u003eBecause of its central role in cholesterol metabolism and inflammation, MVK is a crucial target for understanding metabolic regulation and disease pathogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=MVK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=MVK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=MVK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/MVK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=MVK+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047295934829,"sku":"FY12632","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e3c897da-24c9-4ef6-a132-fb289bc7394a.jpg?v=1782237021","url":"https:\/\/www.ebiohippo.com\/products\/mvk-antibody-mevalonate-kinase-bha17135535","provider":"BioHippo","version":"1.0","type":"link"}